不同剂量碘海醇对大鼠肾脏氧自由基的影响

目的:探讨不同剂量碘海醇对大鼠肾脏氧自由基的影响,为造影剂的安全使用提供依据。方法:将40只Wister大鼠随机分为成4组:空白对照组(A)、小剂量碘海醇组(B)、中剂量碘海醇组(C)、大剂量碘海醇组(D)。B、C、D组尾静脉注射碘海醇,24h后检测血清中肌酐(Scr)水平、肾组织中丙二醛(MDA)含量及超氧化物歧化酶(SOD)活力,显微镜下观察肾脏病理改变。结果:A、B、C、D四组血清中Cr水平分别为(46.21±8.25)、(60.54±13.34)、(80.16±15.26)、(94.27±16.81)μmol/L,肾脏组织MDA含量分别为(2.74±0.48)、(4.51±1.47)、(6.92±2.18)、(8.87±2.31)nmol/mg蛋白,SOD活力分别(195.21±11.01)、(170.24±13.59)、(140.86±25.91)、(121.54±25.50)U/mg蛋白,各组间差异有统计学意义(P<0.05)。结论:肾脏内氧自由基随着碘海醇剂量的增加而增加,呈正相关。

The Effects of Different Doses of Iohexol on Oxygen Free Radicals of Kidney in Rats

Objective：To investigate the effects of different doses of iohexol injected on oxygen free radicals of kidney in rats. Methods：A total of 40 rats were randomly divided into 4 groups： blank control group（ A）, small dose of iohexol group（ B）, moderate dose of iobexol group（ C）, large dose of iohexol group（D）. After 24 h blood samples were collected for measurement of serum ereatinine（Cr） level. The rats were sacrifieod and the renal tissues were removed for determination of MDA content and SOD activities. Pathological changes in renal tissues were observed with light microscopes. Results ：The concentrations of serum Cr in group A, B, C and D were （46.21 ±8.25）, （60.54 ±13.34） ,（80.16 ±15.26）, （94.27 ± 16.81） μmol/L. The content of MDA in renal tissues were （2.74 ± 0.48）, （4.22 ± 1.40）, （6.15 ± 1.88）, （8.07 ± 2.21 ） nmol/mg protein, and activities of SOD were （ 195.21 ± 11.01 ）, （ 175.64 ± 13.43）, （ 146.36 ± 21.11 ）, （ 125.57 ± 22.55） U/mg protein. Difference between the four groups was statistically significant（ P 〈 0.05 ）. Conclusion：The oxygen free radicals in the kidney increase with the increase of the dose of iohexol. There are positively correlation between them.