涎腺腺样囊性癌细胞株p16基因缺失、突变及表达意义

目的　研究 p16基因在涎腺腺样囊性癌细胞株中的缺失和突变等结构变化及其表达之间的关系。方法　应用聚合酶链反应 (PCR)和 PCR-单链构象多态性 (SSCP)对涎腺腺样囊性癌细胞株 ACC- 2和高转移细胞克隆 ACC- M进行 p16基因缺失、突变的检测 ,应用免疫组化方法检测 p16基因在细胞株中的蛋白表达。结果　涎腺腺样囊性癌细胞株 ACC- 2检测 p16基因阳性 ,高转移细胞克隆 ACC- M缺失 ,两个细胞克隆均无点突变 ,ACC- 2的p16蛋白表达阳性 ,而 ACC- M蛋白表达阴性。结论　 p16基因在高转移涎腺腺样囊性癌克隆中的缺失 ,表明 p16基因在涎腺腺样囊性癌的演进和转移中具有抑癌作用。

STUDY ON DELETION AND MUTATION EXPRESSION OF P16 GENE IN HUMAN SALIVARY CYSTIC CARCINOMA CELL LINE

Objective To investigate the deletion and mutation of p16 gene in human salivary cystic carcinoma (ACC) and the relationship between the structural alteration of p16 gene and its expression. Method PCR assay was used to detect deletion of p16 gene in cell line ACC 2 and high metastatic cell strain ACC M that cloned from ACC 2. PCR SSCP assay was used to detect mutation of p16 gene in ACC 2 and ACC M. Immumohistochemistry method was used to detect the protein expression of p16 in ACC 2 and ACC M. Results Deletion was found in ACC M by PCR method. There was no mutation in the two cell line and clone by PCR SSCP method. Positive staining was found in ACC 2 and negative staining was found in ACC M by immumohistochemistry method. Conclusion The result demonstrate that deletion of p16 gene and no expression of its protein in high metastatic cell strain ACC M play an important role in suppressing the progress and metastasis of ACC.