Two Extramitochondrial Circular DNA Species in the Petite Negative Yeast Schizosaccharomyces pombe: Relative abundance and size determination by electron microscopy |
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| Authors: | F Manna L Del Giudice W H Schreil K Wolf |
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| Institution: | (1) Istituto Internazionale di Genetica e Biofisica, Consiglio Nazionale delle Ricerche, Via G. Marconi 10, 80125 Napoli, Italy;(2) Institut für Genetik und Mikrobiologie der Universität München, Maria Ward Straße 1a, 8000 München 19, Germany |
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| Abstract: | Summary In this paper we present the electron microscopic analysis of two distinct extramitochondrial circular DNA species in the fission yeast Schizosaccharomyces pombe (S. pombe). Both DNA species can be isolated from mitochondrial fractions, but disappear after DNase treatment of mitochondria, demonstrating their extramitochondrial location. The size of these molecular species is 3.08 ± 0.18  m and 2.00 ± 0.09 m (standard deviation). They are present in a ratio of approximately 9:1 in the DNA preparations analyzed. |
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| Keywords: | Extramitochondrial DNA S pombe |
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