罗布麻颗粒对化疗型卵巢早衰大鼠Bax和Bcl-2蛋白表达的影响

目的： 探讨罗布麻颗粒对化疗型卵巢早衰大鼠Bax 和Bcl-2 蛋白表达的影响。方法：性成熟SD雌鼠随机分 为对照组、造模组，腹腔注射（ 顺铂1.5 mg/kg）制备卵巢早衰模型，随机分为卵巢早衰模型组和罗布麻颗粒高、 中、低剂量治疗组。H-E 染色观察卵巢形态；ELISA 检测各组大鼠血清Bax、Bcl-2 和雌二醇、促黄体生成素（LH）、 促卵泡激素（FSH））的含量，免疫组织化学法检测卵巢Bax 与Bcl-2 蛋白表达。结果：卵巢早衰模型组大鼠中初 级卵泡减少，闭锁卵泡增多，罗布麻颗粒高剂量治疗组大鼠可见初级卵泡，闭锁卵泡较卵巢早衰模型组减少；卵 巢早衰模型组大鼠血清中雌二醇及Bcl-2 含量降低，FSH、LH、Bax 的含量升高； 各药物治疗组雌二醇、Bcl-2 含 量升高，FSH、LH、Bax 的含量降低；Bax 蛋白主要表达在颗粒层细胞及卵泡液中，卵巢早衰模型组大鼠较对照 组表达显著升高， 各药物治疗组在不同程度上能够降低其表达；Bcl-2 蛋白在各组主要表达在颗粒层细胞中，卵 巢早衰模型组大鼠较对照组表达显著下降， 各药物治疗组在不同程度上能够升高其表达。结论：顺铂能够导致雌 性大鼠血清中性激素水平的紊乱及卵巢组织形态的改变，可能与细胞凋亡因子Bax 与Bcl-2 在血清及卵巢组织中 表达水平的高低有关；罗布麻颗粒可能通过下调Bax 蛋白、上调Bcl-2 蛋白从而改善顺铂致卵巢早衰大鼠的卵巢 功能。

Effect of apocynum venetum granule on Bax and Bcl-2 protein expression in rats with premature ovarian failure induced by chemotherapy

Objective To investigate the effect of apocynum venetum granule on the expression of Bax and Bcl-2 protein in rats with chemotherapy-induced premature ovarian failure. Methods The model of premature ovarian failure （POF） was established by intraperitoneal injection of 1.5 mg/kg cisplatin. The rats were randomly divided into POF model group， high-dose， medium dose and low-dose apocynum venetum treatment groups. The morphology of the ovary was observed by H-E staining ； the levels of Bax， Bcl-2， estradiol （E2）， luteinizing hormone （LH） and follicle stimulating hormone （FSH） in serum were detected by ELISA ； the protein expressions of Bax and Bcl-2 in the ovary were detected by immunohistochemistry. Results The number of primary follicles decreased and the number of atresia follicles increased in the POF model group， and the number of atresia follicles decreased in the high-dose apocynum venetum granule treatment group compared with the POF model group. The content of E2 and Bcl-2 in the serum of the POF model group decreased， and the content of FSH， LH and Bax increased. Bax protein was mainly expressed in granulosa cells and follicular fluid， and the expression of Bax protein was significantly higher in POF model group than that in control group. Bcl-2 protein was mainly expressed in granulosa cells. Compared with the control group， the expression in POF model group decreased significantly， and the drug treatment increased the expression in different degrees. Conclusion Chemotherapy drug cisplatin can cause the disorder of serum sex hormone level and the change of ovarian tissue morphology in female rats， the possible reason is related to the expression level of apoptosis factor Bax and Bcl-2 in serum and ovarian tissue. Apocynum venetum granule may improve ovarian function of rats with premature ovarian failure induced by cisplatin by down regulating Bax protein and up-regulating Bcl-2 protein.