小鼠4倍体胚胎诱导蜕膜化反应

目的 探讨小鼠囊胚内细胞团(ICM)在蜕膜化过程中是否具有调控作用.方法 采用电融合技术制备4倍体胚胎,作为内细胞团缺陷胚胎,通过输卵管胚胎移植制备4倍体胚胎诱导蜕膜组织,以正常2-细胞胚胎诱导的蜕膜组织作为对照,从着床位点和蜕膜形态对两组蜕膜进行比较分析后,采用高通量测序筛选两组中差异表达的微小RNA(miRNA),...

Decidualization induced by mouse tetraploid embryos

Objective To investigate the role of inner cell mass(ICM)in decidualization using decidua induced by two-cell embryos or tetraploid embryos through tubal embryo transfer.Methods Tetraploid embryo,as the inner cell mass-deficient embryo,were produced by electrofusion of mouse 2-cell embryos.Decidua was induced by 2-cell embryos or tetraploid embryos through tubal embryo transfer.Decidua induced by 2-cell embryos was employed as a control.Morphologic and implantation site of decidua were compared between two-cell embryo-induced decidua and tetraploid embryo-induced decidua.The differentially expressed microRNA(miRNA)was screened by high-throughput sequencing.The target genes of differentially expressed miRNA in two groups were screened for Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG).Results Tetraploid embryo-induced decidual tissue and 2-cell embryoinduced decidual tissue were very similar at the implantation site,but there were significant differences in decidual morphology.There were 16 miRNAs differentially expressed in decidua of the two groups,of which 11 miRNAs(miR-466f-3p,miR-302d-3p,miR-466i-5p,miR-465c-5p,miR-302a-5p,miR-7068-3p,miR-741-3p,miR-302a-3p,miR-433-5p,miR-144-5p,miR-878-5p)were up-regulated in tetraploid embryo-induced decidua and 5 miRNAs(miR-690,miR-193b-5p,miR-147-3p,novel 327,miR-363-3p)were down-regulated in tetraploid embryo-induced decidua.Bioinformatics analysis showed that the target genes played function such as protein binding and ion binding,and mainly involved in cyclic guanosine monophosphate-protein kinase G(c GMP-PKG)signaling pathway,estrogen signaling pathway,vascular endothelial growth factor(VEGF)signaling pathway and so on.Conclusion ICM plays an important role in decidualization.