胺碘酮对大鼠睾丸形态和功能的影响及其作用机制

目的:探讨胺碘酮对大鼠睾丸质量、形态、酶活性及精子活力的影响.方法:健康雄性SD大鼠随机分为对照组、低剂量组和高剂量组,连续灌胃方式给予胺碘酮4周.处死大鼠后,分离大鼠双侧睾丸,并称重;光学显微镜观察精子活力,H-E染色观察睾丸形态;采用乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)、一氧化氮合酶(NOS)及一氧化氮(N...

Effects and related mechanism of amiodarone on testicular morphology and function in rats

Objective To investigate the effects of amiodarone on testicular weight， testicular morphology， testicularenzyme activity and sperm motility in rats. Methods SD rats were randomly allocated into control group， low-dosegroup and high-dose group and continuously gavaged with amiodarone for 4 weeks. When the rats were sacrificed， thetestes of the rats were quickly separated and weighed ；testicular morphology and sperm mobility were observed bylight microscopy ； lactate dehydrogenase （LDH）， succinate dehydrogenase （SDH） and nitric oxide synthase （NOS）activities and nitric oxide （NO） levels were determined by LDH， SDH， NOS and NO assay kits， respectively.Results The rats in the high-dose group showed listlessness， gray hair， decreased activity and increased aggressivebehavior. Part of ektexine of the seminiferous tubule of the testes of the rats in the high dose group was folded， andthe spermatogenic epithelial cells were irregularly arranged. In terms of testicular quality， testicular LDH activityand SDH activity， there were no statistically significant differences between the low-dose group and the high-dosegroup and the control group. In terms of NOS activity， NO content and sperm motility in testicular tissue， there wereno statistically significant differences between the low-dose group and the control group， but there were statisticallysignificant differences between the high-dose group and the control group. Conclusion High-dose amiodarone causesirregular arrangement of seminiferous tubules in the testis， increase of NOS activity and NO content in the testis，decline of sperm motility， which indicates that high-dose amiodarone may have an impact on male testis and affectmale reproductive function.