糖尿病病人红细胞变形能力与脂质过氧化损伤及血浆sC5b-9浓度的关系

1目的　探讨糖尿病病人红细胞变形能力 (ED)与红细胞膜脂质过氧化物 (L PO)及血浆补体激活片段 (s C5 b- 9)浓度变化的关系。2方法　采用 DXC- 30 0 A核孔滤膜红细胞变形能力测定仪、硫代巴比妥酸比色法和酶联免疫吸附法 (EL ISA) ,检测了 79例糖尿病病人红细胞滤过指数 (EFI)、红细胞膜 L PO和 s C5 b- 9浓度变化。3结果　糖尿病病人 EFI,L PO和 s C5 b- 9明显高于对照组 ,差异有显著性 (t=6 .2 5 0～ 2 6 .119,P<0 .0 0 1)。有血管病变者各指标增高较无血管病变者更明显 (t=8.82 5～ 2 0 .0 0 0 ,P<0 .0 0 1)。糖尿病病人和有血管病变者 EFI与 L PO和s C5 b- 9呈正相关 (r=0 .714,0 .779,P<0 .0 0 1)。4结论　糖尿病病人 ED降低与红细胞膜 L PO和血浆 s C5 b- 9浓度增高有密切关系。

THE RELATIONSHP BETWEEN THE ERYTHROCYTE DEFORMABILITY AND LIPID PEROXIDATIVE INJURY AND PLASMA sC5b-9 IN DIABETIC PATIENTS

Objective To explore the relationship between erythrocyte deformability(ED) and erythrocyte membrane lipid peroxidase (LPO), plasma concentration of complement activation composition(sC5b 9) in diabetic patients.\ Methods\ The erythrocyte filtration index(EFI),LPO and sC5b 9 were measured in 79 diabetic patients by using apparatus,thiobarbituric acid and enzyme linked immunosorbent assay(ELISA) methods.\ Results\ The EFI, LPO and sC5b 9 were significantly higher in diabetic patients than those in controls( t=6.250-26.119,P <0.001),and the increase of EFI, LPO and sC5b 9 was more obvious in diabetic patients with angiopathy than that in diabetic patients without angiopathy( t=8.825-20.000,P <0.001). The EFI was positively correlated with LPO,sC5b 9 in diabetic patients and patients with angiopathy ( r=0.714,0.779,P <0.001).\ Conclusion\ The decrease of ED in diabetic patients was related to the increase of erythrocyte membrane LPO and plasma sC5b 9 concentrtion.