天花粉(栝楼)标准汤剂的UPLC指纹图谱研究

目的:建立天花粉(栝楼)标准汤剂超高效液相色谱指纹图谱,为其质量控制提供科学、合理的方法。方法:CORTECS T3色谱柱(150 mm×2.1 mm,1.6μm),甲醇-0.1%磷酸溶液为流动相梯度洗脱。检测波长为220 nm;流速为0.2 mL·min^-1;柱温为30℃。结果:建立了天花粉(栝楼)标准汤剂的指纹图谱,标示出4个共有峰,并对15批天花粉(栝楼)标准汤剂UPLC指纹图谱进行相似度评价,与对照指纹图谱相似度均在0.90以上。结论:该方法简单、准确、重复性好,可为制定天花粉配方颗粒的质量控制标准提供参考。

Study on UPLC Fingerprint Analysis of Trichosanthis(Trichosanthes kirilowii)Standard Decoction

Objective:In order to fully reflect the features of quality control,the fingerprint analysis method of trichosanthis(trichosanthes kirilowii)standard decoction was established.Methods:CORTECS T3 column(150 mm×2.1 mm,1.6μm)was used with the column temperature maintained at 30℃.The mobile phase was methanol-0.1%phosphoric acid solution for gradient elution.The detection wavelength was at 220 nm and the flow rate was 0.2 mL/min.Results:The fingerprint spectrum of trichosanthis standard decoction was obtained,and four common peaks were identified.The similarity of fingerprint spectrum among 15 batches of standard decoction was also evaluated.Conclusion:The fingerprint analysis method of trichosanthis standard decoction)is simple,accurate and reproducible,which provides a scientific reference for establishing the quality control specification of trichosanthis formula granule.