自由基诱导剂对氟中毒大鼠脑氧化水平的影响

目的 观察脑组织中脂质过氧化物含量和脂肪酸组成的变化。方法 将３６只Ｗｉｓｔａｒ大鼠分成３组,分别饲以相同饲料、不同浓度含氟水,５个月后处死。用铁离子诱导氟中毒大鼠脑组织脂质过氧化反应,用高效液相色谱方法测定脑组织中丙二醛含量,用氧电极方法测定脂质过氧化过程中的氧耗量,用气相色谱方法测定脑组织中各类脂肪酸组成。结果 用自由基诱导剂激发脂质过氧化后,与对照组动物相比,氟中毒动物脑组织中丙二醛明显增多

Influence of free radical inducer on the level of oxidative stress in brain of rats with fluorosis

OBJECTIVE: To study changes in content of lipid peroxide and composition of fatty acids in the brain of rats affiliated with chronic fluorosis after treatment with free radical inducer (ferric ion). METHODS: Thirty-six Wistar rats were divided into three groups, fed with similar fodder and varied concentrations of fluoride in drinking water, and were killed five months after treatment. Lipid peroxidation was induced by ferric ions. Malondialdehyde content in brain was analysed by high-performance liquid chromatography; oxygen consumption was determined with an oxygen electrode and fatty acid composition was measured by gas chromatography in brain tissues of the rats. RESULTS: In the brain tissues, content of malondialdehyde and oxygen consumption increased, composition of polyunsaturated fatty acids decreased and that of saturated fatty acids decreased after treatment with free radical inducer in the treated group, as compared with those in control group. CONCLUSION: Over uptake of fluoride for a long term could cause potential increase in the level of oxidative stress in the brain tissue.