| 复方苦参液对大肠癌裸鼠皮下移植瘤的体外实验研究 |
| |
| 引用本文： | 徐月,秦剑,周远大,何海霞.复方苦参液对大肠癌裸鼠皮下移植瘤的体外实验研究[J].第四军医大学学报,2009,30(4):316-319. |
| |
| 作者姓名： | 徐月 秦剑 周远大 何海霞 |
| |
| 作者单位： | 徐月,XU Yue(重庆市中医研究院肛肠外科);秦剑,QIN Jian(重庆市中药研究院化学研究室);周远大,何海霞,ZHOU Yuan-Da,HEI Hai-Xia(重庆医科大学第一附属医院临床药理学研究室,重庆,400016)
|
| |
| 摘 要： | 目的：探讨复方苦参液对大肠癌裸鼠皮下移植瘤的抑瘤情况及相关作用机制。方法：30只裸鼠建立大肠癌皮下移植瘤动物模型，随机分为3组：空白对照组（注射生理盐水）、低浓度治疗组和高浓度治疗组.观察3组第3，7，14，21日皮下移植瘤生长情况及裸鼠体质量的变化；治疗结束时取肿瘤组织及心、肝、肺、肾等行病理组织学检查，免疫组化法检测增殖细胞核抗原（PCNA）和CD34，TUNEL法检测细胞凋亡情况。结果：复方苦参液对裸鼠移植瘤的生长有明显抑制作用，与对照组相比，瘤体质量明显降低、体积减小（P〈0.05），坏死面积显著增加，细胞增殖降低（P〈0.05），血管生成减少（P〈0.05），凋亡指数明显升高（P〈0.05），但对裸鼠体质量无明显影响，心、肝、肺、肾等组织未见明显病理学改变。结论：复方苦参液具有抑制入大肠癌裸鼠皮下移植瘤生长的作用，可能与其抑制血管生成和诱导细胞凋亡有关。
|
| 关 键 词： | 结直肠肿瘤 苦参碱 小鼠 裸 增殖细胞核抗原 细胞凋亡 |
Study of matrine injections on colorectal carcinoma cell line LoVo transplanted subcutaneously in nude mice |
| |
| XU Yue,QIN Jian,ZHOU Yuan-Da,HEI Hai-Xia.Study of matrine injections on colorectal carcinoma cell line LoVo transplanted subcutaneously in nude mice[J].Journal of the Fourth Military Medical University,2009,30(4):316-319. |
| |
| Authors: | XU Yue QIN Jian ZHOU Yuan-Da HEI Hai-Xia |
| |
| Institution: | XU Yue1,QIN Jian2,ZHOU Yuan-Da3,HEI Hai-Xia31Department of Enterochirurgia,2Laboratory of Chemistry,Academy of Chinese Materia Medica,3Basic Medicine Project Research Lab,First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China |
| |
| Abstract: | AIM: To investigate the effect and the mechanism of matrine injections on the growth of human colorectal carcinoma cell line LoVo transplanted subcutaneously in nude mice.METHODS: Nude mouse model of human colorectal carcinoma by subcutaneous transplantation of LoVo cell line was reproduced.A total of 30 mice were divided into 3 groups: control group,low dosage group and high dosage group.The growth of the subcutaneously transplanted tumor and changes in mouse body weight in each group after treatment were ... |
| |
| Keywords: | colorectal neoplasms matrine mice nude proliferating cell nuclear antigen apoptosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
