Major histocompatibility complex class II alleles in an Uygur population in the Silk Route of Northwest China |
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| Authors: | N Mizuki S Ohno H Ando T Sato T Imanishi T Gojobori M Ishihara K Goto M Ota Z Geng L Geng G Li H Inoko |
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| Institution: | Department of Ophthalmology, Yokohama City University School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236, Japan;Department of Genetic Information, Division of Molecular Life Science, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259–11, Japan;Japanese Red Cross, Kanagawa, Shonan Blood Center, 1837 Aiko, Atsugi, Kanagawa 243, Japan;Department of Immunotransplantation, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259–11, Japan;Center for Information Biology, National Institute of Genetics, Mishima, Shizuoka 411, Japan;Department of Legal Medicine, Shinshu University School of Medicine, 3–1–1 Asahl, Matsumoto, Nagano 390, Japan;Institute of Genetics, Fudan University, Shanghai 200–433, China;Department of Biology, Xining University, Ürümqi, China |
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| Abstract: | Abstract: HLA class II (DRB1, DQA1, DQB1 and DPB1) genotyping was performed in 57 unrelated Uygur individuals inhabiting the northwestern China area by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Among 98 DRBI alleles tested, 23 alleles were detected, and DRB1*0701 (16.7%) and DRB1*0301 (14.0%) were the most and the second most common alleles, respectively. In 8 DQA1 alleles detected, DQA1*05 (26.3%), DQA1*03 (21.9%) and DQA1*0201 (21.1%) were very frequent. Of 21 DQB1 alleles tested, 13 were observed. Among them, DQB1*02 was highly predominant with the gene frequency of 32.5%. Of 46 DPB1 alleles tested, 15 were detected, among which DPB1*0401 (31.6%) was the most frequent. Two haplotypes predominate clearly; DRB1*0701-DQA1*0201-DQB1*02 (15.5%) and DRB1*0301-DQA1*05-DQB1*02 (12.6%). The dendrogram constructed by the neighbour-joining (NJ) method based on the allele frequencies of the DRB1, DQA1, DQB1 and DPB1 genes of 13 representative populations all over the world suggested that Uygur belonged to the Asian group and lay at the closest genetic distance to a Kazak population inhabiting the same area. |
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| Keywords: | HLA genotyping Uygur PCR-RFLP haplotype anthropology Silk Route |
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