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| 版纳微型猪近交系133家系SLA-DQ cDNA的克隆和序列分析 | |
| 引用本文： | 曾嵘,苗永旺,霍金龙,潘伟荣,曾养志. 版纳微型猪近交系133家系SLA-DQ cDNA的克隆和序列分析[J]. 实验动物与比较医学, 2005, 25(4): 215-221 |
| 作者姓名： | 曾嵘 苗永旺 霍金龙 潘伟荣 曾养志 |
| 作者单位： | 1. 山东省疾病预防控制中心,济南,250014 2. 山东中医药大学附属医院,济南,250011 3. 山东省中医药研究院,济南,250014 4. 山东省实验动物中心,济南,250015 |
| 基金项目： | 山东省自然基金资助项目（编号：Y2002C37） |
| 摘 要： | 目的 建立小鼠在体肿瘤多药耐药模型。方法 模拟临床以亚于治疗剂量的联合化疗PFC方案，诱导S180腹水型小鼠4周，流式细胞仪荧光检测P170、LRP、TOPOⅡ，建立能客观反应临床肿瘤多药耐药产生机制并适合中药干预肿瘤多药耐药研究的在体细胞模型。结果 化疗诱导后昆明小鼠S180腹水肿瘤细胞P170、LRP的表达率和ROPOⅡ活性明显提高，经传代后其表达稳定。结论 联合化疗诱导建立小鼠在体肿瘤多药耐药模型是可行的。 |
| 关 键 词： | 肿瘤 多药耐药 小鼠 P170 LRP TOPO Ⅱ |
| 文章编号： | 1004-8448(2005)04-0215-03 |
| 收稿时间： | 2005-06-06 |
| 修稿时间： | 2005-06-06 |
Moleculor Cloning and Characterization of SLA-DQ cDNA of the Banna Mini-Pig Inbred Line Family 133 |
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| ZENG Rong,MIAO Yong-wang,HUO Jin-long,PAN Wei-rong,ZENG Yang-zhi. Moleculor Cloning and Characterization of SLA-DQ cDNA of the Banna Mini-Pig Inbred Line Family 133[J]. Laboratory Animal and Comparative Medicine, 2005, 25(4): 215-221 | |
| Authors: | ZENG Rong MIAO Yong-wang HUO Jin-long PAN Wei-rong ZENG Yang-zhi |
| Affiliation: | 1. Shandong Center for Disease Control and Prevention, Jinan 250014, China; 2. Teaching Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China; 3. Shandong Academy of TCM, Jinan 250014, China; 4. Shandong Provincial Center of Laboractory Animal, Jinan 250015, China |
| Abstract: | Objective To establish the mice model of multidurg resistance of tumorous expression in ascites with S180 cell. Methods The mice with S180 cell in ascites were treated with the method of PFC of repeated-combined-chemotherapy in less man the therapy dose for 4 weeks. The levels of P170 and LRP and TOPOII were detected by Flow Cytometry Analysis. Results The cellular multidurg rdsistance of tumorous expression (P170 and LRP) and the activity of TOPOII of mice were improved obviously and the expression was stable in the cell line. |
| Keywords: | Tumor Multidurg resistance of tumorous Mice P170 TOPOII |
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