三酰甘油与总胆固醇试剂对总胆汁酸测定的干扰

目的通过实验研究三酰甘油和总胆固醇试剂对总胆汁酸（TBA）测定的干扰及消除。方法以Randox质控血清为样本，分别单独测定TBA，测定三酰甘油（TG）、总胆固醇（TC）后测定TBA和经过不同的清洗程序、测定通道后测量TBA并测量TG、TC试剂中的TBA含量。结果先测量TG、TC后再测量TBA．其均值分别为（64．33±3．22）μmol／L和（56．34±3．56）μmol／L，与对照组的（20．34±4．66）μmol／L相比，差异有统计学意义（P〈0．01）；TG经过特殊清洗及增加比色杯和试剂针清洗后，TBA测量值与正常比较无区别（P〉0．05）；TC经过特殊清洗后，仍高于对照（P〈0．05），但增加比色杯和试剂针清洗后，TBA测量值与正常比较无区别（P〉0．05）；先进行TC、TG测量后连续10次TBA含量，可以看到前6次结果与对照组之间差异有统计学意义（P〈0．05），从第7次开始，差异无统计学意义（P〉0．05），但测量值仍有轻微区别；TC、TG试剂中TBA含量高于生理盐水（P〈0．01）。结论TC、TG试剂中含有高剂量TBA，可能对试剂针、比色杯造成污染；仪器清洗和改变测量通道可以抵抗这种污染，但真正的消除还需依赖技术的进步。

Interference of TG and TC on determination of TBA

Objective To study the interference and elimination of TBA after measuring of TG and TC. Methods Separately measured the concentration of TBA on Randox control serum after TG and TC;After different cleaning pro- cedures and different channel measured concentration of TBA. Results After measured TC and TG,the concentration of TBA were （64.33±3.22） μmol/L and （56.34±3.56） μmol/L,higher than the control group（20.34±4.66）μmol/L （P 〈 0.01）; After special cleaning,needle cleaning and cup cleaning of TC,TBA was no different with control group （P 〉 0.05）; After special cleaning of TG, TBA was still higher than control group （P 〈 0.05）, but cleaning the cup and needle, con- centration of TBA was normal （P 〉 0.05）; 10 consecutive TBA,the 7 and after were still higher than control but statis tic difference disappear （P 〉 0.05）; TBA in TC and TG reagent content were higher than normal saline （P 〈 0.01）. Conclusion TC,TG reagent containing high doses of TBA which may pollute the needle and cup; Machine cleaning and channels changing can resist this pollution,but the real elimination depends on the progress of technology.