| 新疆哈萨克族、锡伯族人群D16S539、D7S820和D13S317位点多态性比较研究 |
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| 引用本文： | 陈玲,许健,黄辰,袁育康,宋土生,李生斌. 新疆哈萨克族、锡伯族人群D16S539、D7S820和D13S317位点多态性比较研究[J]. 中国优生与遗传杂志, 2001, 9(6): 12-13 |
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| 作者姓名： | 陈玲 许健 黄辰 袁育康 宋土生 李生斌 |
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| 作者单位： | 1. 石河子医学院免疫学教研室,
2. 西安医科大学, |
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| 基金项目： | 本研究属国家自然科学基金项目39670399,国家"95"攻关项目96-919-01-04-3 |
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| 摘 要： | 采用复合PCR扩增，6％变性聚丙烯酰胺凝胶电泳结合银染技术首次对我国新疆哈萨克族（n=102)和锡拍族（n=110）群体进行D16S539、D7S820、D13S317三个STR位点的调查，建立了这两个民族群体遗传学数据库。经统计学检验，三个位点的基因型频率分布符合Hardy-Weinberg平衡定律。结果显示，哈萨克族群体中观察到8、7、8种等位片段，24、20、23种基因型。杂合率为：0．8019－0．8259；而锡伯族群体中则观察到7、8、8种等位片段，22、20、21种基因型，杂合度为：0．88750．9286。此外，比较两个民族的多态性分布，发现在D7S820位点上无统计学意义（P＞0．05），而其他两们位点均可见显著性差异P＜0．05）。三个STR位点在两个民族中的综合检验对于民族起源、变迁和民族识别以及民族间亲缘关系研究提供了较高的价值。
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| 关 键 词： | PCR STR 哈萨克族 锡伯族 |
| 修稿时间： | 2000-12-11 |
Study on polymorohisms of three STR Loic in the Kazak and Xibo ethnic population |
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| Chen Xueling,Xu Jian,et al.. Study on polymorohisms of three STR Loic in the Kazak and Xibo ethnic population[J]. Chinese Journal of Birth Health & Heredity, 2001, 9(6): 12-13 |
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| Authors: | Chen Xueling Xu Jian et al. |
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| Abstract: | A Chinese Kazak(n=102) and Xibo (n=110) ethnic populatin in XinJiang were investigated at three STR loci (D16S539,D7S820,D13S317) by multiplex amlification,6% denaturing PAGE and silver staining.8,7,8 alleles and 24,20,23 genotypes were observed at these 3 STR loci respectively in Kazak ethnic population;7,8,8 alleles and 22,20,21 genotypes were observed in Xibo ethnic population.The heter ozygosities for the three loci were 0.8019-0.8259 and 0.8875-0.9286 at two ethnic population respectively.The genotypes distributions were in accordance with Hardy-Weinberg equibrium.In addition,There were significant difference of allelic distribution between the Kazak and Xibo ethnic population but at D7S820(P<0.05).The polymorphisms of three STR loci has practical value in the origin and change of different ethnic population and their consanguinity. |
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| Keywords: | PCR STR Kazak Xibo |
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