氧化苦参碱对小鼠淋巴结T细胞增殖的双向作用

目的检测氧化苦参碱(Oxymatrine,OMT)对刀豆蛋白A(Con A)刺激的小鼠淋巴结T细胞增殖的影响,探讨OMT对免疫系统的作用机制,为临床用OMT治疗免疫相关性疾病提供理论和实验依据.方法利用CFDA-SE染色,流式细胞术检测淋巴细胞在多克隆刺激剂Con A和OMT的共同作同下荧光强度的变化,并应用CELLQuest软件分析OMT对小鼠淋巴结T细胞增殖的影响程度.结果 500、125和31μg/mLOMT对小鼠淋巴结T细胞增殖呈剂量依赖性抑制,而16、8、4、2μg/mL OMT对小鼠淋巴结T细胞增殖起促进作用,但其剂量依赖关系不明显.结论 CFDA-SE染色和流式细胞术是分析淋巴细胞增殖的有力工具:OMT对小鼠淋巴结T细胞的增殖呈双向作用.

Two-ways effects of Oxymatrine on T lymphocyte proliferation in mouse lymph node stimulated by Con A

[Objective] To investigate the effects of Oxymatrine (OMT) on mouse lymphocyte proliferation stimulated by Con A, explore the mechanism of the effects of OMT on the immune system and provide theoretical and experimental evidence for the clinical application of OMT in treating immune-related diseases. [Methods] CFDA-SE staining and flow cytometry were used to detect the fluorescence intensity of lymphocytes after stimulated by polyclonal stimulators Con A and OMT. Related softwares were used to analyze the effects of OMT on mouse lymphocyte proliferation. [Results] OMT has the function to restrain the proliferation of lymphocyte of mouse depended on its concentration with 500, 125 and 31 μg/mL in substrate, but 16, 8, 4, 2 μg/mL concentration, it improves the proliferation of T lymphocytes of mouse's lymph node, the dependence on its concentration is not significant. [Conclusions ] 1. Both CFDA-SE dyeing and flow cytometer were reliable tools to analyze lymphocyte proliferation. 2. OMT has the two-ways effects on T lymphocyte proliferation in mouse lymph node stimulated by Con A.