COMPARISON OF CULTURED HUMAN MONONUCLEAR CELLS, LIMULUS AMEBOCYTE LYSATE and RABBITS IN THE DETECTION OF PYROGENS |
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| Authors: | E. Wind Hansen J. Dencker Christensen |
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| Affiliation: | Royal Danish School of Pharmacy, Department of Biological Sciences, Copenhagen. |
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| Abstract: | Isolated human mononuclear cells exposed to either lipopolysaccharide or Staphylococcus aureus secreted interleukin-1 like material. The secretion was concentration dependent. The minimal detectable level in the test solution of lipopolysaccharide and Staphylococcus aureus was 200 pg/ml and 10(5) cells/ml respectively. The sensitivity and specificity of the Limulus Amebocyte Lysate test, the rabbit pyrogen test and the monocyte test are compared. The monocyte test is proposed as an alternative in-vitro test to the rabbit pyrogen test. |
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