| 大鼠胶质瘤细胞系C6趋化因子受体CXCR4和甲酰肽受体FPR的表达 |
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| 引用本文： | 陈剑鸿,卞修武,杨世昕,王吉民. 大鼠胶质瘤细胞系C6趋化因子受体CXCR4和甲酰肽受体FPR的表达[J]. 第三军医大学学报, 2005, 27(17): 1722-1724 |
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| 作者姓名： | 陈剑鸿 卞修武 杨世昕 王吉民 |
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| 作者单位： | 第三军医大学西南医院病理科,重庆,400038;Laboratory of Molecular Immunoregu-lation Center for Cancer Research,National Cancer Institute at Frederick,National Institute of Health,USA |
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| 基金项目： | 国家自然科学基金，国际抗癌联盟ICRETT基金 |
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| 摘 要： | 目的观测大鼠源性胶质瘤细胞系C6趋化细胞因子受体CXCR4和甲酰化肽受体(formyl peptide receptor,FPR)的表达水平并探讨其与瘤细胞分化和血管生成的关系.方法传代培养大鼠源性胶质瘤细胞系C6,采用免疫细胞化学方法检测CXCR4和FPR表达,并对细胞分化标记物胶质纤维酸性蛋白(GFAP)和波形蛋白(vimentin)以及血管内皮生长因子(VEGF)表达水平进行检测.结果C6细胞呈长梭形,突起细长,GFAP表达较强,vimentin呈强表达.该细胞高表达CXCR4和FPR,并呈VEGF强阳性.结论C6细胞分化程度低,其高表达的趋化因子受体CXCR4和FPR可能与胶质瘤血管生成有密切关系.
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| 关 键 词： | 胶质瘤 化学趋化因子受体 细胞分化 血管生成因子 |
| 文章编号： | 1000-5404(2005)17-1722-03 |
| 收稿时间： | 2004-07-25 |
| 修稿时间： | 2004-11-15 |
Expression of chemokine CXCR4 and formyl peptide receptor FPR in rat C6 glioma cell line |
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| CHEN Jian-hong,BIAN Xiu-wu,YANG Shi-xin,WANG Ji-min. Expression of chemokine CXCR4 and formyl peptide receptor FPR in rat C6 glioma cell line[J]. Acta Academiae Medicinae Militaris Tertiae, 2005, 27(17): 1722-1724 |
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| Authors: | CHEN Jian-hong BIAN Xiu-wu YANG Shi-xin WANG Ji-min |
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| Affiliation: | 1.Department of Pathology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China; 2.Laboratory of Molecular Immunoregulation,Center for Cancer Research, National Cancer Institute at Frederik, National Institute of Health, USA |
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| Abstract: | Objective To detect the expression of chemoattractant receptors, CXCR4 and formyl peptide receptor (FPR), in rat glioma cell line C6 and to explore their relation to tumor differentiation and angiogenesis. Methods The expression of CXCR4 and FPR by C6 cells were detected with immunocytochemistry. The differentiatial degrees were also detected to analyze the relationship of chemoattractant receptors with angiogenesis and malignancy of glioma. Results C6 cells were of a long spindle shape, expressing high level of vimentin but relatively low level of glial fibrillary acidic protein (GFAP). C6 cells were positive for CXCR4 and FPR in the cytoplasm and strongly positive for vascular endothelial growth factor (VEGF). Conclusion C6 cell line is poorly differentiated and highly expresses chemoattractant receptors CXCR4 and FPR, both of which might be related to the angiogenic potentials. |
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| Keywords: | glioma chemoattractant receptor cell differentiation angiogenic factor |
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