基质金属蛋白酶-9及基质金属蛋白酶组织抑制物-1与卵巢上皮性癌血管生成的关系

目的:探讨基质金属蛋白酶-9(MMP-9)及基质金属蛋白酶组织抑制物-1(TIMP-1)在卵巢上皮性癌血管生成中的作用。方法:采用免疫组织化学SP法检测45例卵巢上皮性癌组织中MMP-9和TIMP-1的表达,并检测其中微血管密度(MVD,CD34标记)。结果:卵巢癌组织和卵巢良性肿瘤中MMP-9阳性表达率明显高于正常卵巢组织(P<0.01),而TIMP-1未见升高(P>0.05),并且TIMP-1在卵巢癌组织中的阳性表达率显著低于MMP-9(P<0.01)。MMP-9在卵巢癌组织中表达与MVD显著正相关(r=0.286,P<0.05),而TIMP-1与MVD无相关性(r=0.194,P>0.05)。卵巢癌中MMP-9表达高于TIMP-1者,其MVD显著高于MMP-9表达低于TIMP-1者(P<0.05)。结论:MMP-9和TIMP-1表达失衡可能在卵巢上皮性癌血管生成中起重要作用,MMP-9表达增强而TIMP-1表达降低,其血管生成能力可能显著增强,但并非唯一决定因素。检测卵巢上皮性癌中MMP-9和TIMP-1表达对进一步了解卵巢上皮性癌局部血管生成情况有一定应用价值。

The relationship between the expression of MMP-9, its inhibitor TIMP-1 and angiogenesis in invasive ovarian epithelial carcinoma

Objective:To investigate the role of matrix metalloprotein-9 (MMP-9) and its inhibitor matrix metalloprotein-1(TIMP-1) in regional tumor angiogenesis in ovarian epithelial carcinoma.Methods:The expression of MMP-9, TIMP-1 and microvessed density (MVD) labeled by CD34 were detected by immunohistochemistry SP methods in 75 cases with early invasive ovarian epithelial carcinoma, 18 cases with benign ovarian tumor and 15 cases with human nomal ovary.Results:The positive expression rates of MMP-9 in human nomal ovary and benign ovarian tumor were significantly higher than that in ovarian epithelial carcinoma (P<0.05).The positive expression rate of TIMP-1 in benign ovarian tumor was significantly lower than that of MMP-9 (P<0.01).There was positive correlation between MVD and MMP-9 in benign ovarian tumor (γ=0.286,P<0.05), but there was no positive correlation between MVD and TIMP-1 (γ=0.194,P>0.05).The level of MVD in the cases with higher expression rate of MMP-9 and lower expression rate of TIMP-1 was significantly higher than that in the cases with lower expression rate of MMP-9 and higher expression rate of MMP-1(P<0.05).Conclusion:In invasive ovarian epithelial carcinoma, unbalanced expression of MMP-9 and MMP-1 may play an important role during tumor angiogenesis. High expression of MMP-9 and low expression of TIMP-1 may result in a remarkably development of tumor angiogenesis, but not the only decisive factor. Detection of MMP-9 and TIMP-1 may be valuable to further understanding of reginal tumor angiogenesis in invasive ovarian epithelial carcinoma.