| RP-HPLC法测定人血浆中盐酸氨溴索的浓度 |
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| 引用本文： | 孙亚欣,肇丽梅,邱枫,何晓静.RP-HPLC法测定人血浆中盐酸氨溴索的浓度[J].实用药物与临床,2009,12(3). |
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| 作者姓名： | 孙亚欣 肇丽梅 邱枫 何晓静 |
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| 作者单位： | 中国医科大学附属盛京医院临床药理研究室,沈阳,110004 |
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| 摘 要： | 目的建立RP-HPLC测定人血浆中盐酸氨溴索含量的方法。方法选用Hypersil-BDS C18柱(5.0mm×150mm,粒径5μm),流动相:乙腈-pH值7.5磷酸钠缓冲液(65∶35),流速:0.9mL/min,检测波长:248nm,柱温:25℃。结果该方法低、中、高3种浓度的提取回收率为85.2%～97.7%,日内、日间相对标准差为0.77%～12.2%(n=6),在20～640ng/mL范围内线性关系良好。结论本法简便、灵敏、专属性强,能满足含量测定及体内药代动力学研究的需要。
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| 关 键 词： | 盐酸氨溴索 高效液相色谱 血药浓度 |
Determination of ambroxol hydrochloride in humam plasma by RP-HPLC |
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| SUN Ya-xin,ZHAO Li-mei,QIU Feng,HE Xiao-jing.Determination of ambroxol hydrochloride in humam plasma by RP-HPLC[J].Practical Pharmacy and Clinical Remedies,2009,12(3). |
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| Authors: | SUN Ya-xin ZHAO Li-mei QIU Feng HE Xiao-jing |
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| Abstract: | Objective To develop a fast reverse-phase high-performance liquid chromatography assay method for the determination of ambroxol hydrochloride.MethodsA Hypersil-BDS C18(5.0 mm×150 mm,5 μm)column was used.The mobile phase consisted of acetonitrile-phosphate buffer(35∶65).Detection wavelength was 248 nm.ResultsA good linear relationship was obtained in the concentration range of 20～640 ng/mL.The average recovery of this method was 85.2%～97.7%.The RSD of within-day and between-day were all less than 15%.ConclusionThe method is simple,rapid and accurate.The result showed that the method was suitable for the determination of ambroxol hydrochloride. |
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| Keywords: | Ambroxol hydrochloride RP-HPLC Plasma concentration |
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