| 蛋白质转移电泳技术在单克隆抗体检测中的应用 |
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| 引用本文： | 李俐,杨翰仪.蛋白质转移电泳技术在单克隆抗体检测中的应用[J].吉林大学学报(医学版),1987(3). |
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| 作者姓名： | 李俐 杨翰仪 |
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| 作者单位： | 白求恩医科大学生化教研室
(李俐)，白求恩医科大学生化教研室(杨翰仪) |
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| 摘 要： | 抗原(粗制)经聚丙烯胺凝胶电泳分离后,将凝胶上的蛋白质条带经电泳转移到硝酸纤维素膜上。在硝酸纤维素膜上抗原与加入的相应单克隆抗体结合,再与辣根过氧化物酶标记的第二抗体反应。然后通过酶对底物的催化作用,在抗原位置上出现棕褐色条带,证明了单克隆抗体与相应抗原的特异结合。
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| 关 键 词： | 转移电泳 单克隆抗体 硝酸纤维素膜 |
Protein transfer electrophoresis for immunological screening of monoclonal antibodies |
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| Li Li and Yang Hanyi.Protein transfer electrophoresis for immunological screening of monoclonal antibodies[J].Journal of Jilin University: Med Ed,1987(3). |
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| Authors: | Li Li and Yang Hanyi |
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| Institution: | Li Li and Yang Hanyi |
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| Abstract: | After electrophoretic transfer of antigens from polyacrylamide gel tonitrocellulose, the membrane was used for determining the specificity of themnnoclonal antibodies against thyroglobin by ELISA.The immunological activity ofthemonoclonal antibodies can be tested and verified on the blot by the appearancerown bands at the position of the corresponding antigens. |
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| Keywords: | Protein transfer electrophoresis Monoclonal antibodies Nitrocellulose |
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