应用引导组织再生膜与人工骨、碱性成纤维细胞生长因子等生物材料治疗牙周骨缺损

背景：引导组织再生已成功地应用到牙周病的治疗，应用生物相容性膜，依靠其机械屏障作用能阻止牙龈上皮根向生长，并形成一定的间隙以利于牙周膜细胞占据根面，进而重建牙周结构。 目的：验证引导组织再生是否可促进牙周骨缺损的修复及增加新骨的形成，将引导组织再生和人工骨，生长因子等生物材料联合使用是否可获得更多牙周新组织。 设计、时间及单位：动物实验观察， 2006-02/2006-07在大连大学医学实验中心完成。 材料：取碱性成纤维细胞生长因子冻干粉 (50 mg/支)，ddH20彻底溶解，制成含碱性成纤维细胞生长因子 5 g/L的溶液。将胶原膜剪为10 mm ×10 mm大小，取1 mL溶液滴注胶原膜上完全复合，使每张膜含5 mg 碱性成纤维细胞生长因子。 方法：刮取16只大耳白兔兔牙骨质，去骨高度为自牙槽脊水平至釉牙骨质界下6 mm制成缺损，右侧用加入碱性成纤维细胞生长因子的人工骨和引导组织再生含生长因子的引导组织再生膜包绕缺损，全瓣复位、缝合，作为实验组，左侧作为对照，制备方法及大小同，骨缺损直接全瓣复位、缝合。 主要观察指标：光镜下观察载体材料吸收情况，结缔组织内炎症情况，结合上皮根向移行情况。 结果：对照侧均呈现牙槽骨不愈合现象，实验侧有不同程度的牙槽骨骨愈合现象。与对照比较，实验组愈合效果较好。 结论：引导组织再生膜与人工骨、碱性成纤维细胞生长因子等生物材料膜技术的应用有利于骨缺损的愈合。

Guided tissue regeneration membrane, artificial bone, and basic fibroblast growth factors for treatment of periodontal bone defects

BACKGROUND: Guided tissues regeneration (GTR) has been successfully used in the treatment of periodontal diseases by using biocompatible membrane to prevent tooth epithelial cell growing towards root and to facilitate the formation of a certain gap which is favorable for periodontal membrane cells to occupy the surface of the root, thereby to rebuild the peridental structure. OBJECTIVE: To verify whether GTR can promote the repair of periodontal bone defect and the formation of new bones and whether combined use of GTR membrane, artificial bone, and growth factors can acquire much more new periodontal tissue. DESIGN, TIME AND SETTING: An animal observation experiment was performed at the laboratory of Medical College of Dalian University between February and July 2006. MATERIALS: Basic fibroblast growth factor (bFGF) freeze-dry powder (50 mg/ampoule) was thoroughly dissolved with ddH2O to prepare 5 g/L bFGF solution. bFGF solution was dropped onto collagen membranes with a size of 10 mm ×10 mm ( 5 mg bFGFs per piece of collagen membrane). METHODS: Cementum and the alvelor bone 6 mm below the cement-enamel junction were removed to induce periodontal defect in 16 rabbits with big ears. Artificial bone with bFGF and GTR membrane were used to fold around the defect on the right side, followed by reduction and suture, serving as experimental side. The left side underwent identical experimental procedure, with the exception of application of artificial bone with bFGF and GTR membrane, serving as control side. MAIN OUTCOME MEASURES: Carrier material absorption, inflammation of inner connective tissues, and status of junctional epithelium were examined through the use of microscope. RESULTS: The control side exhibited no concrescence, while the experimental side displayed concrescence of the cementum to various extents. CONCLUSION: Application of GTR membrane, artificial bone, and bFGF can promote the concrescence of damaging bone.