p53和C-erbB-2在非洲地方性和AIDS相关Kaposi肉瘤中表达的意义

目的 :探讨p5 3蛋白、p5 3mRNA和C erbB 2蛋白表达在非洲地方性和AIDS相关Kaposi肉瘤中表达的意义。方法 :用免疫组织化学和原位杂交方法 ,对 30例地方性和AIDS相关Kaposi肉瘤中p5 3和C erbB 2的表达作了标记 ,用图像分析仪对表达的阳性程度作了定量测定。根据Kaposi肉瘤组织中血管成分和梭形细胞的比例分为血管瘤样型、梭形细胞型和混合型。结果 :p5 3蛋白在血管瘤样型表达弱 ,梭形细胞型表达强 ,差异有极显著性(P <0 .0 1) ,p5 3mRNA表达在血管瘤样型高于梭形细胞型 ,两者差异有极显著性 (P <0 .0 1)。C erbB 2蛋白在梭形细胞型的表达高于血管瘤样型 (P <0 .0 5 )。结论 :p5 3蛋白、p5 3mRNA和C erbB 2蛋白的异常表达在Kaposi肉瘤的进展过程中发挥了重要作用

Significance of p53 and C-erbB-2 expression in endemic and AIDS-related Kaposi' s sarcoma in Africa

Purpose:To study the significance of p53 protein, p53mRNA, and C erbB 2 protein expressions in Africa endemic and AIDS related Kaposi's sarcoma (KS).Methods:Immunohistochemistry and hybridization in situ were performed with p53 and C erbB 2 in 30 cases with endemic and AIDS related KS.The degree of pasitivity of p53 and C erbB 2 in KS tissues were measured with image analysis system. KS was classified into three types, i.e., angiomatous type, spindle cell type, and mixed type according to the proportion of blood vessel components and spindle cells in the lesions.Results:The degree of positivity of p53 protein was stronger in spindle cell type than that in angiomatous type ( P <0.01), whereas the expression of p53 mRNA was stronger in angiomatous type than that in spindle cell type ( P <0.01). The expression of C erbB 2 protein in spindle cell type was stronger than that in angiomatous type ( P <0.05).Conclusions:The changes of p53 protein, p53mRNA, and C erbB 2 protein in KS have played an important role in the development of endemic and AIDS related KS in Africa.