交联温度对京尼平交联壳聚糖/藻酸盐组织工程支架的影响

目的]通过对壳聚糖/藻酸盐复合支架材料表面结构、降解率、细胞毒性、孔隙率、含水量以及生物力学性能变化的研究,探究交联温度对京尼平交联壳聚糖/藻酸盐组织工程支架的影响.方法]运用冷冻干燥法制备壳聚糖/藻酸盐支架材料,分别在4℃、25℃、37℃条件,于0.5％京尼平溶液中交联24 h,作为实验组.以未用京尼平交联的支架材料作为对照组,评价支架材料降解率、细胞毒性、孔隙率、含水量以及生物力学性能的特点.结果](1)伴随交联温度升高,支架材料的颜色从浅紫色变为紫黑色,未交联的支架材料为白色；(2)降解率4周后在4℃为21.54％±3.07％、25℃组为9.9％±1.2％、37℃组为8.98％±0.79％.其中,37℃和25℃组抗降解能力优于4℃组(P＜0.05).各实验组均优于对照组(P＜0.01)；(3)弹性模量在4℃组为0.84±0.55,25℃组为1.44±0.06,37℃组为1.53±0.02,对照组为0.79±0.16.对照组与25℃组及37℃组之间差异有统计学 意义(P＜0.05)；(4)孔隙率、含水量、细胞毒性各实验组间未见明显差异.结论]随着交联温度的提高,支架材料的抗降解能力、抗拉伸性能增加,且对支架材料的结构、细胞毒性、孔隙率以及含水量无明显影响.

Effect of temperature on cross-linking of chitosan/alginate scaffolds with genipin

Objective] Through research of the change of the chitosan/alginate scaffolds material surface structure,degradation rate,cytotoxicity,porosity,water content and biomechanics properties,to explore cross-linking temperature of genipin crosslinked effects of chitosan/alginate scaffolds for tissue engineering.Method]The chitosan / alginate scaffolds of tissue engineering was made by freeze-drying,respectively,in 4 ℃,25 ℃,37 ℃ conditions,at 0.5% solution of genipin crosslinked 24 h,as the experimental group.The scaffolds of not cross-linked with genipin as a control group to evaluate the degradation rate of scaffolds,cytotoxicity,porosity,water content and biomechanics properties.Result](1)The color of scaffolds material changed from light purple to purplish black,according with the rise of the cross-linking temperature,however,not cross-linked scaffold material was white.(2)The degradation rate after 4 weeks at 4℃ group was 21.54%±3.07%,25℃ group was 9.9%±1.2%,37℃ group was 8.98%±0.79%.Among them,the 37℃ group of degradation resistant capacity was better than the 4℃ group(P<0.05).The experimental groups were better than the control group(P< 0.01).(3)Elastic modulus at 4 ℃ group was 0.84±0.55,25℃ group was 1.44±0.06,37℃ group was 1.53±0.02.37℃ group and 25℃ group of tensile properties better than control group(P<0.05).(4)Porosity,water content,cytotoxicity between the experimental groups had no significant difference.Conclusion]With the cross-linking temperature increasing,scaffold degradation resistant capacity and tensile properties were strengthen,but on the scaffold material structure,cytotoxicity,porosity and water content were not altered significantly.