葛根中葛根黄酮的水提取及大孔树脂纯化工艺研究

目的 优选葛根中总黄酮的水提取及大孔树脂的纯化工艺.方法 以葛根素得率和葛根总黄酮得率为指标,采用正交试验法优选葛根水提工艺,单因素考察优选葛根水提液的大孔树脂纯化工艺.结果 葛根的最佳水提工艺为9倍量水提取3次,每次90 min,葛根总黄酮和葛根素提取率分别为92.17%和84.91%.葛根水提液的最佳大孔树脂富集工艺为:HPD-750大孔树脂,径高比1∶ 6,上样液浓度1.5 g/mL,最大上样量3 BV,上样流速1 mL/min,洗脱剂70%乙醇,洗脱剂用量5 BV.葛根水提液经大孔树脂纯化后的出膏率为4.47%,纯化物中葛根总黄酮和葛根素的含量分别为76.22%和56.20%.结论 该工艺操作简便易行,适用于大规模生产.

Optimization of water extraction and macroporous resin purification processes of flavonoids from Radix Puerariae

Objective To optimize the processes of water extraction and macroporous resin purification of flavonoids from Radix Pueraiae. Methods The orthogonal test was used to optimize the water extraction process and the monofactorial observation to optimize the macroporous resin purification process, with the extraction rates of puerarin and the total flavonoids as the indexes. Results The optimum water extraction process was as follows: 9 times mount of water was used to extract pueraria for 3 times with 90 min each time. The extraction rate of the total flavonoids was 92.17% and that of puerarin, 84.91%. The optimum macroporous resin purification process was as follows: macroporous resin HPD-750 was used with the ratio of diameter to length as 1:6, the concentration of the sample solution was 1.5 g/mL, the maximum sample was 3 BV, the flowing rate was 1 mL/min, the eluant was 70% ethanol and the eluant volume was 5 BV. The extract yield was 4.47% after macroporous resin purification. The content of total flavonoids was 76.22% and that of puerarin, 56.20%. Conclusion This optimum process is easy to operate, which can be applied in large-scale producution of the total flavonoids and puerarin.