| 提高悬浮细胞脂质体转染效率的方法 |
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| 引用本文： | 苑晓玲,从玉文,善亚君,陈家佩. 提高悬浮细胞脂质体转染效率的方法[J]. 军事医学科学院院刊, 2002, 26(1): 56-56,78 |
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| 作者姓名： | 苑晓玲 从玉文 善亚君 陈家佩 |
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| 作者单位： | 军事医学科学院放射医学研究所,北京,100850 |
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| 摘 要： | 目的：提高悬浮细胞脂质体转染效率。方法：将细胞分成6组，脂质体组、纤维蛋白（fibronectin)＋脂质体组、电击＋脂质体组、纤连蛋白＋电击＋脂质体组、辐射＋脂质体组、纤维蛋白＋辐射＋脂质体组。各实验组均转染EGFP－N1质粒。计算转染效率。结果和结论：脂质体与其他条件组合使用，可以较好地提高悬浮细胞转染效率。
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| 关 键 词： | 脂质体 纤维蛋白 基因治疗 悬浮细胞脂质体 转染效率 |
| 文章编号： | 1000-5501(2002)01-0056-02 |
Methods to improve the liposome transfection efficiency of suspended cells |
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| YUAN Xiao_Ling,CONG Yu_Wen,SHAN Ya_Jun,CHEN Jia_Pei. Methods to improve the liposome transfection efficiency of suspended cells[J]. Bulletin of the Academy of Military Medical Sciences, 2002, 26(1): 56-56,78 |
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| Authors: | YUAN Xiao_Ling CONG Yu_Wen SHAN Ya_Jun CHEN Jia_Pei |
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| Abstract: | Objective:To improve transfection efficiency of suspended cells.Methods:In order to know the efficiency of different liposome transfection methods,the cells were divided into six groups:GeneJammer;fibronection plus GeneJammer;electricity plus GeneJammer;fibronectin plus electricity and GeneJammer;radiation plus GeneJammer;fibronection plus radiation and GeneJammer.All groups were transfected by plasmid EGFP_N1.The transfection efficiency was computed.Results and Conclusions:The transfection efficiency was improved when GeneJammer was used in combinction with other factors. |
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| Keywords: | cell transfection suspensions liposome fibronectin |
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