肉苁蓉对败血症大鼠肝线粒体能量代谢功能的影响

目的研究肉苁蓉(Cistanche deserticola Y.C.Ma)对败血症大鼠肝线粒体功能的影响,明确其具体作用机制。方法采用盲肠结扎穿孔术(CLP)复制败血症模型,采用生理多导仪监测平均动脉压(MAP)的变化,定磷法测定肝线粒体ATP酶活性,采用Clark氧电极法测定呼吸控制率(RCR),Ⅲ态的变化。结果大鼠在CLP术后,线粒体ATP酶活性,RCR和Ⅲ态显著降低(P<0.01)。肉苁蓉给药组肝线粒体ATP酶活性,RCR和Ⅲ态较对照组明显改善(P<0.05),与大鼠死亡率的降低呈显著正相关。结论肉苁蓉能增强实验性败血症大鼠线粒体能量代谢功能。

Effect of Cistanche deserticola Y. C. Ma on Energy Metabolic Function of Liver Mitochondria Following Sepsis

Objective To study the effect of Cistanche deserticola Y.C.Ma on energy metabolic function of liver mitochondria following sepsis and investigate the treatment mechanism.Methods The model of sepsis was made by cecal ligation and puncture. Cistanche deserticola Y.C.Ma and saline were administrated by gastric garage to the rats respectively for fifteen days.Liver mitochondria RCR,ATPase activity were assayed.Results Liver mitochondria RCR ,ATPase activity were obviously decreased after CLP.Compared with control group, Cistanche deserticola Y.C.Ma could obviously reversed the changes(P<0.01). Furthermore the effect was positive correlated to the improvement of the death rate.Conclusion Cistanche deserticola Y.C.Ma can strengthen the energy metabolic function of liver mitochondrial,effectively attenuate the dysfunction of hepatocytic mitochondria from the damage induced by sepsis.