强肌健力方对脾肾两虚大鼠细胞因子水平的影响

目的：观察强肌健力方对实验性脾肾两虚证大鼠血清细胞因子含量变化的影响,探讨脾肾两虚证与细胞因子的关系,以期进一步了解该方药对改善脾肾两虚证的意义及黄芪在方中的作用。方法：采用随机法将72只SD大鼠分为五组,正常对照组10只,脾肾两虚组16只,强肌健力方组15只,黄芪减量组15只,黄芪组16只。造模分两个阶段完成。第1d～14d采用大黄造模;第15d～24d采用氢化可的松复制脾肾两虚证大鼠模型。应用大黄及氢化可的松造模,分别给予蒸馏水、强肌健力方、黄芪减量方,黄芪方灌胃,连续给药24d后,采用放射免疫分析观察各组动物血清IL-2、IL-6、TNF水平的变化。结果：脾肾两虚组大鼠血清IL-2、TNF含量比正常对照组低（P〈0.05～0.01）,而IL-6含量则升高（P〈0.01）。强肌健力方组能升高IL-2、TNF含量和降低IL-6含量（P〈0.01）。黄芪减量组能使TNF含量升高及降低IL-6含量。黄芪组能使IL-6含量降低,但对IL-2、TNF含量没有影响。结论：脾肾两虚证的发生与细胞因子网络调节系统的失衡有关,强肌健力方可以改善脾肾两虚证大鼠这种免疫功能失衡,通过健脾益气功效来调节细胞因子水平,从而达到增强免疫功能的作用。这为脾肾两虚证时体内细胞因子水平的改变提供了理论依据,同时也提示该方重用黄芪可能是防治脾肾两虚的一个关键。

Effect of Qiangji Jianli Fang on the Serum Levels of Cytokines (IL-2, TNF, IL-6) in Rat Models of Spleen-kidney Deficiency Syndrome

Objective To study the therapeutic effect of Qiangji Jianli Fang（QJJLF）on the changes of serum cytokines levels（IL-2,TNF,IL-6）in rat models of spleen-kidney deficiency syndrome and the role played by Astragalus as a component of QJJLF.Methods Rat models of spleen-kidney deficiency syndrome were established with daily gavage with rhubarb for 14 days followed by daily intramuscular hydrocortisone（25mg/μg）for 10 days.The models were divided into the following groups ① no treatment,n=10 ② treated with daily QJJF 2ml/d,n=14 ③ treated with daily QJJLF with the strength of Astragalus reduced,n=14 ④ treated with Astragalus only,n=14.Serum levels of IL-2,TNF and IL-6 were determined with RIA on d25 in all the models.Ten normal rats served as controls.Results The general condition of the models without any treatment was very poor,being feeble anorexic and sluggish in movement.The treated models were much better.Serum levels of IL-2 and TNF in the no-treatment group were significantly lower than those in controls（P0.05~0.01）but the IL-6 levels were significantly higher（P0.01）.In the models treated with QJJLF,the serum levels of IL-2 and TNF were significantly higher and levels of IL-6 significantly lower than those in the no-treatment group（P0.05~0.01）.In the models treated with QJJLF（with reduced strength of Astragalus,serum levels of TNF were significantly higher and IL-6 levels significantly lower than those in no-treatment group,but the serum IL-2 levels were only insignificantly increased.In the models treated with Astragalus only,the IL-6 levels were significantly lower than those in the no-treatment models,but changes of TNF and IL-2 were in significantly.Conclusion QJJLF could improve the general condition as well as correct the abnormal IL-2,TNF and IL-6 serum levels in rat models of spleenkidney deficiency syndrome with improvement of the immuno-function.Astragalus was a very important ingredient in the formula of QJJLF.