番茄原汁对人前列腺癌PC-3细胞生长抑制作用的研究

目的　探讨番茄原汁对人前列腺癌PC -3细胞生长抑制作用及其可能机制。方法　不同浓度的番茄原汁作用于人前列腺癌PC- 3细胞4 8小时;采用噻唑蓝(MTT)法检测番茄原汁对人前列腺癌PC- 3细胞的生长抑制作用,彗星试验检测PC -3细胞DNA损伤作用。结果　番茄原汁能抑制人前列腺癌PC- 3细胞生长,与对照组相比差异有极显著性;引起PC-3细胞DNA单链断裂,出现拖尾的彗星细胞,拖尾率与尾长随着番茄原汁浓度增大而增加,表现明显的剂量反应关系。结论　番茄原汁对PC- 3细胞生长抑制作用的机理可能与其诱导人前列腺癌PC-3细胞DNA损伤的有关

Study on the inhibitory effects of juice of tomato on the growth of human prostate carcinoma PC-3 cells

OBJECTIVE: To study on the inhibitory effects of juice of tomato on the growth of human prostate carcinoma PC-3 cells and its possible mechanism. METHODS: PC-3 cells were treated with the juice of tomato in different concentration (40, 80, 120 ul/ml) for 48h; the proliferation of PC-3 cells were measured by MTT assay, the comet assay was used to measure the DNA damage of PC-3 cells. RESULTS: Juice of tomato could inhibit the proliferation of PC-3 cells, the growth inhibitory rate of experimental groups were significantly higher than that of control group, with very statistical significance; and it could induce the breakage of DNA single strand of PC-3 cells and resulted in comet cells with tail, Rate of DNA tail and the tail length of DNA increased with the increasing of concentration of juice of tomato, showing the obvious dose effect relationship. CONCLUSION: Juice of tomato could lead to DNA damage of PC-3 cells, it was related to that could inhibit the proliferation of PC-3 cells.