| 阿糖胞苷对子宫颈癌细胞的端粒酶活性的影响 |
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| 引用本文： | 黄浩,方向明,胡继军.阿糖胞苷对子宫颈癌细胞的端粒酶活性的影响[J].武汉大学学报(医学版),2004,25(4):456-458. |
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| 作者姓名： | 黄浩 方向明 胡继军 |
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| 作者单位： | 武汉市第一医院实验中心,武汉,430022;武汉市第三医院,武汉,430060 |
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| 摘 要： | 目的 :探讨阿糖胞苷对体外培养的宫颈癌细胞 (Hela细胞 )端粒酶活性的影响。方法 :采用PCR ELISA法检测经阿糖胞苷处理前后的Hela细胞株端粒酶活性的改变。结果 :经阿糖胞苷处理后 ,Hela细胞的生长受到抑制 ,端粒酶活性明显降低。结论 :阿糖胞苷对Hela细胞具有生长抑制作用 ,其抗肿瘤作用的机理可能与抑制肿瘤细胞的端粒酶活性有关。
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| 关 键 词： | 阿糖胞苷 宫颈癌细胞 端粒酶 |
| 文章编号： | 1671-8852(2004)04-0456-03 |
| 修稿时间： | 2003年5月29日 |
Effect of Ara-c on the Telomerase Activity of Hela Cells |
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| Huang Hao,Fang Xiangming,Huo JijunCenter of Experimental Medicine,First Hospital of Wuhan,Wuhan ,China.Effect of Ara-c on the Telomerase Activity of Hela Cells[J].Medical Journal of Wuhan University,2004,25(4):456-458. |
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| Authors: | Huang Hao Fang Xiangming Huo JijunCenter of Experimental Medicine First Hospital of Wuhan Wuhan China |
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| Institution: | Huang Hao,Fang Xiangming,Huo JijunCenter of Experimental Medicine,First Hospital of Wuhan,Wuhan 430022,China |
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| Abstract: | Objective: To investigate effects to telomerase activity by Ara-c in Hela cells in vitro . Methods: MTT assay was used to investigate the inhibitory effect of Ara-c on Hela cell in vitro . The telomerase activity in cells treated by Ara-c was detected by PCR-ELISA assay which was based upon PCR amplification of the initial telomerase product and detected by ELISA. Results: The growth of Hela cells was inhibited by Ara-c and the telomerase activity in Hela cells was significantly reduced. Conclusion: The Ara-c could directly inhibit the growth of Hela cells. The mechanism of anti-tumor by Ara-c may be explained by the inhibition of telomerase activity in cells by Ara-c. |
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| Keywords: | Ara-c Hela cells telomerase |
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