水飞蓟宾—磷脂酰胆碱复合物对糖尿病大鼠肾脏的保护作用

目的探讨水飞蓟宾—磷脂酰胆碱复合物(SPC)对糖尿病大鼠肾脏的保护作用。方法SD大鼠50只,适应性喂养1周后,随机选10只作为对照组,其余40只腹腔注射链脲佐菌素(STZ)60 mg.kg-1,72 h后测空腹血糖,将血糖值>16.7 mmol.L-1的大鼠33只随机分为模型组、SPC高剂量组和SPC低剂量组,每组11只,对照组和模型组每日灌胃质量分数1%的羧甲基纤维素钠(CMC-Na)混悬液,SPC高、低剂量组分别灌胃给予SPC(用质量分数1%CMC-Na配成混悬液)300 mg.kg-1、150 mg.kg-1,连续12周,第12周末称体质量、心脏穿刺取血,分离血清检测血清中肌酐(Cr)、尿素氮(BUN)、血清果糖胺(FMN),同时取左肾测肾系数,取右肾测超氧化物歧化酶(SOD)、丙二醛(MDA)和晚期糖基化终末产物(AGEs)。结果与对照组比较,模型组大鼠体质量明显下降,肾脏系数明显升高(P<0.001);SPC各剂量组与模型组比较体质量明显升高,肾脏系数明显下降(P<0.05);与对照组比较,模型组大鼠血清FMN、Cr、BUN及肾组织中的AGEs值、MDA值明显升高,SOD活力明显下降(P<0.001);SPC各剂量组与模型组比较,血清中的FMN、Crea、BUN及肾组织中的AGEs值、MDA值均明显下降(P<0.05),SOD活力明显升高(P<0.05)。结论SPC对糖尿病大鼠肾脏具有一定的保护作用。

Protective effect of silybin-phosphatidylcholine compound on kidney in rats with diabetes

Objective To investigate the protective effect of silybin-phosphatidylcholine compound （SPC） on kidney in STZ-induced diabetic rats. Methods Fifty SD rats were fed for one week, then ten rats were randomly chosed as control group,the other forty rats were injected with 60 mg · kg^-1 streptozotocin （STZ） through enterocoelia respectively. The levels of blood-fasting sugar of the rats were measured after 72 hours,the thirty-three rats whose blood glucose were higher than 16.7 mmol · L^-1 were randomly divided into three groups ： model group, high dose SPC group and minidose SPC group （ n = 11 ）. The rats in normal control and model groups were administered with 1% sodium carboxymethyl cellulose （CMC-Na） suspension for 12 weeks, and two SPC groups were respectively administered with 300 mg · kg^-1 and 150 mg · kg^-1 SPC mixed with 1% CMC-Na suspension for 12 weeks. At the end of 12 week ,the levels of serumal creatinine（Cr） ,blood urea nitrogen（BUN） and fructosamine（FMN） were detected, at the same time, the levels of MDA, SOD and advanced glycation endoproducts （AGEs） in the nephridial tissue of cortex renis were detected. Results Compared with control group, the weights of diabetic rats were decreased and the kidney indexes were increased in model group（ P 〈 0.001 ）. The weights of diabetic rats in different doses of SPC groups were obviously increased and the kidney indexes were decreased than those in model group （ P 〈 0.05 ）. Compared with control group, the serum levels of Cr, BUN, FMN and MDA in nephridial tissue were obviously increased, SOD activity were obviously decreased in model group（ P 〈 0.001 ）, and in SPC groups ,these indexes were obviously altered than those in model group（ P 〈 0.05 ）. Conclusion SPC possesses potential protective effect on kidney in rats with diabetes.