20(S)-人参皂苷Rg3对前列腺癌PC-3M细胞的诱导凋亡作用

目的研究20(S)-人参皂苷Rg3(SPG-Rg3)对人前列腺癌PC-3M细胞诱导凋亡的作用,并探讨其可能机制。方法采用MTT法观察SPG-Rg3对PC-3M细胞生长的抑制作用,计算IC50;用流式细胞术测定PC-3M细胞周期的变化;AO/EB双染观察SPG-Rg3对PC-3M细胞凋亡的形态学变化;利用免疫细胞化学染色和RT-PCR技术探讨SPG-Rg3对PC-3M细胞的诱导凋亡作用及与其相关基因caspase-8的关系。结果SPG-Rg3可明显抑制PC-3M细胞的生长,IC50为(248.5±0.58)mg.L-1;PC-3M细胞的生长周期中S期细胞数增加,G2/M期细胞数则明显减少,且在G1峰前出现明显的凋亡峰;SPG-Rg3作用后PC-3M细胞呈现明显的凋亡改变,且细胞caspase-8mRNA含量增加、表达明显增强。结论SPG-Rg3能诱导前列腺癌PC-3M细胞发生凋亡,其机制可能与其活化caspase-8作用有关。

Study on PCo3M cell apoptosis induced by 20 (s)-ginsendoside Rg3 in prostate carcinoma

Aim To study the apoptosis effect of 20(s)-ginsendoside Rg3(SPG-Rg3) on human prostate carcinoma PC-3M cell and the possible mechanism.Methods MTT assay method was adopted to observe the inhibition of PC-3M cell by SPG-Rg3 and the IC50 was calculated.The change of cell phase was determined by flow cytometry.The apoptosis of cell in morphology was observed by AO/EB fluorescence double-dye technology.The apoptosis was investigated by immunocellularchemistry and the changes of apoptotic gene caspase-8 were investigated by RT-PCR.Results SPG-Rg3 inhibited the proliferation of PC-3M cell,IC50 was 248.5±0.58 mg·L-1,significantly lower than that of the control group.Cell number increased in S phase and decreased in G2/M phase in treatment group.Meanwhile,there was an apoptotic apex before G1 apex.Rg3(SPG-Rg3) promoted PC-3M cell apoptosis and the caspase-8 mRNA level was significantly increased.Conclusion SPG-Rg3 can induce PC-3M cell apoptosis,which may be closely related to the activation of caspase-8.