炭疽杆菌培养上清液抗原的制备及免疫保护效果测定

目的 制备炭疽培养上清液抗原并对其免疫效果进行测定。方法将炭疽A16r菌种接种于培养基中培养，收集无菌滤液，并进行超滤浓缩，经Al（OH），佐剂吸附后，皮下免疫Balb／c小鼠，用炭疽Vollum（s）强毒株腹腔攻击测定其免疫效力。结果制备出具有高保护性的上清液抗原，9h产物对Balb／c小鼠免疫效果良好（94．4％）。结论本研究为进一步研究炭疽疫苗免疫保护机制和炭疽疫苗改进研究打下基础。

Preparation of antigen in culture supernatant of Bacillus anthracis and its immunoprotective efficacy

Objective To prepare aluminum hydroxide-adsorbed supernatant antigen from cultures of Bacillus anthracis A16r strain and measure its immunoprotective efficacy.Methods Strain A16r,a nonencapsulated isolate of B.anthracis,was inoculated into medium,and then cell-free filtrate was harvested.After ultrafiltration concentration,the cell-free filtate was absorbed by aluminum hydroxide gel.The immunoprotective efficacy of the adsorbed antigen was measured through subcutaneous immunization and intraperitoneal challenge of Balb/c mice.A standard spore suspension of the virulent Vollum strain of B.anthracis was employed for challenge of immunized mice.Results Filtrates with high antigen activity were obtained.The products of 9 h had high immunoprotective efficacy in Balb/c mice (94.4%).Conclu-(sion The) present works provide a basis for further research of immune mechanism and development of the anthrax vaccine.