| HPLC同时测定升麻中三个酚酸类成分的含量 |
| |
| 引用本文： | 蔡晓星.HPLC同时测定升麻中三个酚酸类成分的含量[J].中国处方药,2020(3):39-41. |
| |
| 作者姓名： | 蔡晓星 |
| |
| 作者单位： | 高邮市中西医结合医院药剂科 |
| |
| 摘 要： | 目的建立快速测定升麻中咖啡酸、阿魏酸及异阿魏酸含量的HPLC方法。方法色谱柱为C18柱(4.6 mm×250 mm,5μm);流动相:乙腈-0.01%磷酸梯度洗脱;柱温:30℃;检测波长:320 nm;流速:1.0 ml/min。结果咖啡酸、阿魏酸、异阿魏酸分别在5.78~115.6μg/ml(r=0.9998,n=6),4.03~80.60μg/ml(r=1,n=6),16.45~329.00μg/ml(r=1,n=6)范围内线性关系良好,回收率在97.6%~101.5%。结论建立的方法准确可靠,可以用于测定升麻中咖啡酸、阿魏酸、异阿魏酸的含量。
|
| 关 键 词： | 升麻 咖啡酸 阿魏酸 异阿魏酸 |
Simultaneous determination of three Phenolic acids in Cimicifuga by HPLC |
| |
| Authors: | CAI Xiao-xing |
| |
| Institution: | (Department of Pharmacy,Integrated traditional Chinese and Western Medicine Hospital,Gaoyou City,Jiangsu Province,Yangzhou 225600,China) |
| |
| Abstract: | Objective A rapid HPLC method for the determination of caffeic acid,ferulic acid and isoferulic acid in Gastrodia elata was established.Methods The column was C18 column(4.6 mm×250 mm,5μm);mobile phase:acetonitrile-0.01%phosphoric acid gradient elution;column temperature:30℃;detection wavelength:320 nm;flow rate:1.0 ml/min.Results the linear relationship of the caffeic acid in the range of 5.78~115.6μg/ml(r=0.9998,n=6)was good,the linear relationship of the ferulic acid in the range of 4.03~80.60μg/ml(r=1,n=6)was good,the linear relationship of the isoferulic acid in the range of 16.45~329.00μg/ml(r=1,n=6)was good,The recovery was 97.6%~101.5%.Conclusion The method was accurate and reliable,and can be used for the determination of caffeic acid,ferulic acid and isoferulic acid in Cimicifuga. |
| |
| Keywords: | Cimicifuga Caffeic acid Ferulic acid Isoferulic acid |
| 本文献已被 维普 等数据库收录！ |
|
