| HPLC同时测定益精丸中梓醇和阿魏酸的含量 |
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| 引用本文： | 孙莹莹.HPLC同时测定益精丸中梓醇和阿魏酸的含量[J].中国处方药,2020(3):36-37. |
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| 作者姓名： | 孙莹莹 |
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| 作者单位： | 南京中医药大学连云港附属医院药学部 |
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| 摘 要： | 目的建立测定益精丸中梓醇和阿魏酸含量的HPLC方法。方法色谱条件:色谱柱:Atlantis T3 C18色谱柱(4.6 mm×250 mm,5μm);混合流动相:乙腈(A)-0.1%磷酸(B)梯度洗脱;流速:0.8 ml/min;检测波长:210 nm;柱温:32℃。结果梓醇和阿魏酸分别在6.24~124.80μg/ml(r=0.9997,n=6),4.96~99.20μg/ml(r=1.0000,n=6)范围内线性关系良好,平均回收率分别为95.2%、95.3%,RSD分别为1.3%和1.8%。结论该方法简单、快速、准确可靠,可用于测定益精丸中梓醇和阿魏酸含量。
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| 关 键 词： | 益精丸 梓醇 阿魏酸 HPLC |
Simultaneous determination of catalpol and ferulic acid in Yijing Pill by HPLC |
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| Authors: | SUN Ying-ying |
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| Institution: | (Department of Pharmacy,Lianyungang affiliated Hospital,Nanjing University of traditional Chinese Medicine,Lianyungang 222004,China) |
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| Abstract: | Objective A method for simultaneous determination of catalpol and ferulic acid in Yijing Pill was established.Methods chromatographic condition:Chromatographic column:Atlantis T3 C18 column(4.6 mm×250 mm,5μm);Mixed mobile phase:The mobile phase A:acetonitrile,B:0.1%phosphate,gradient elution was performed;the flow rate:0.8 ml/min,detection wavelength:210nm,and column temperature:32℃.Results The linear ranges of catalpol and ferulic acid were 6.24~124.80μg/ml(r=0.9997,n=6),4.96~99.20μg/ml(r=1.0000,n=6),respectively.The average recovery rate(RSD)was 95.2%(1.3%),95.3%(1.8%),respectively.Conclusion The method is simple,fast,accurate and reliable,and can be used to determine the content of catalpol and ferulic acid in Yijing pills. |
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| Keywords: | Yijing Pill Catalpol Ferulic acid HPLC |
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