The C5a receptor antagonist PMX205 ameliorates experimentally induced colitis associated with increased IL-4 and IL-10 |
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Authors: | U Jain TM Woodruff AW Stadnyk |
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Affiliation: | 1.Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada;2.School of Biomedical Sciences, University of Queensland, Brisbane, Qld, Australia;3.Department of Pediatrics, Dalhousie University, Halifax, NS, Canada |
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Abstract: | Background and PurposeAnti-complement therapies have not been advanced for treating the inflammatory bowel diseases (IBDs) despite a growing body of evidence that blocking C5a protects against induced colitis in rodents. The purpose of this study was to further build on this evidence by examining the efficacy, mechanism and specificity of a potent, non-competitive and orally active C5a receptor (CD88) antagonist, PMX205, in the dextran sulphate sodium (DSS) model of murine innate colitis.Experimental ApproachMice with DSS added to their drinking water were orally administered 100 or 200 μg day−1 PMX205 in prophylactic and therapeutic regimens. Clinical illness, colon histology and local generation of inflammatory mediators were measured to evaluate the impact of PMX205 on disease.Key ResultsPMX205 significantly prevented DSS-induced colon inflammation in both regimens, associated with lower pro-inflammatory cytokine production and nitrotyrosine staining in colon sections. Additionally, the levels of anti-inflammatory cytokines IL-4 and IL-10 were increased. PMX205 had no significant effect on C5a levels. The beneficial effect of PMX205 was seen in two strains of mice of differing sensitivities to DSS inflammation, but was inactive in mice lacking CD88.Conclusions and ImplicationsPharmacological inhibition of C5a activity by PMX205 is efficacious in preventing DSS-induced colitis, providing further evidence that targeting CD88 in IBD patients could be a valuable therapeutic option. |
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Keywords: | CD88 complement anaphylatoxin C5a PMX205 DSS IBD |
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