病理性瘢痕组织中角朊细胞的免疫诱导作用

目的　探讨增生性瘢痕 (HS)和瘢痕疙瘩 (Ks) ,角朊细胞 (KC)表达 ICAM- 1和 HL A- DR) ,及≤ 1m o的 HS(HS1) ,2～ 6 m o的 HS(HS2 )和 7～ 2 4mo的 HS(HS3)标本 ,分别作抗 ICAM- 1,HL A- DR,CD3,CD4和 CD8的 Mc Ab的SABC方法免疫组化染色和观察 .结果　各组 KC表达 I-CAM- 1和 HL A- DR抗原的阳性检出率 (% )、CD3+ 细胞密度(cells/ mm2 )及 CD4+ / CD8+比值 (x±s) ,并作统计学处理 .各组标本的 KC表达 ICAM- 1和 HL A- DR抗原的阳性检出率依次为 :Ks- I>Ks- P>Ks- A,HS1>HS2 >HS3(t,P<0 .0 5～0 .0 0 1) .各组 KC表达 ICAM- 1和 HL A- DR的阳性率与组织内浸润的 CD3+细胞密度和 CD4+ / CD8+比值呈明显正相关 (P<0 .0 5～ 0 .0 1,r=0 .2 49～ 0 .32 4) .结论　 KS和 HS的 KC通过表达 ICMA- 1和 HL A- DR抗原 ,诱导 T细胞浸润 ,上调局部免疫炎性反应 ,可能是病理性瘢痕形成的重要因素

Immunoinductive effect of keratinocyte in pathnologic scar

AIM　To investigate the relationship between T lymphocytes and the ICAM-1 and HLA-DR antigen expressed by keratinocytes (KC) of hypertrophic scars and keloids.METHODS　Tissue samples were taken from different parts of keloids (Ks) i.e.invasive part (Ks-1), proliferative part (Ks-P) and aged part (Ks-A), and hypertrophic scars (HS) of different stages, i.e.HS of ≤1 mo.(HS1), HS of 2～6 mo.(HS2) and HS of 7～24 mo.(HS3).Immunohistochemical stains (SABC) were used to examine the positive rate of ICAM-1 and HLA-DR antigen expressed by KC, the density of CD3+ T cell (cells/mm2) and the ratio of CD4+/CD8+ cells.RESULTS　The positive expression rate of ICAM-1 and HLA-DR antigen of KC in each group was as follows: Ks-I>Ks-P>Ks-A, HS1>HS2>HS3 (t, P<0.05～0.01).It showed obuious positive correlation with the density of CD3+ T cell and the ratio of CD4+/CD8+ cells.CONCLUSION　Keratinocytes of Ks and HS, by way of expression of ICAM-1 and MLA-DR antigens, could induce T cell infiltration and up-regulate the local immunologic inflammatory response.It may be an important factor in the formation of Ks and HS.