清除法检测肾病综合征患儿肾小球电荷屏障的变化

目的 观察特发性肾病综合征（ＩＮＳ）患儿肾小球电荷屏障（ＧＣＢ）的变化及尿蛋白定量间关系。方法 用选择抑制剂法分别检测２１例ＩＮＳ患儿和年龄匹配的２９例正常儿（对照组）血、尿胰淀粉酶（ＰＡＭ，分子半径２．９ｎｍ），等电点７．０）血，尿唾液淀粉酶（ＳＡＭ，分子半径２．９ｎｍ，等电点５．９￣６．４）清除率，计算两者比值。结果 ＩＮＳ大量蛋白尿组ＣＰＡＭ／ＣＳＡＭ为１．１８（０．８２￣１．５４），非大量

The detection of glomerular charge-barrier in children with nephrotic syndrome by the clearance method

Objective To observed the alteration of the glomerular charge-barrier (GCB) in children with idiopathic nephrotic syndrome(INS) and relationship between GCB and severity of proteinuria. Mothods The clearance ratios of pancreatic to salivary isoamylase(CPAM/CSAM) were detected in 21 cases with INS and 29 healthy controls by use of a selective inbibitor, then use the clearance ration to show the CCB. Results The ratio of CPAM/CSAM in the cases with massive proteinuria was mean 1. 18 (range 0.82 - l .54), in the cases with no-massive proteinuria was mean 2.06(1 .22 - 3. 14), in healthy controls was 3 .33 (l. 18 - 6.54). There was a significant difference in ratios CPAM/CSAM between three groups. As all cases with INS, the ratio was very significantly negatively correlated with 24-hour urine protein quantity, but not correlated when 24 hour proteinuria was in excess of 50 mg/kg. Conclusion GCB of the cases with INS is reduced, and of the cases with massive proteinuria almost dissappeared.