小蓟中蒙花苷对照品的制备和鉴定

目的 研究灰毡毛忍冬的主要药效，并与忍冬相关药效进行比较。方法 通过测定灰毡毛忍冬的最小抑菌浓度（MIC）和最小杀菌浓度（MBC），研究其对6种常见致病菌的抗菌作用；采用醋酸致小鼠腹腔毛细血管通透性增加、二甲苯致小鼠耳廓肿胀、角叉菜胶致大鼠足肿胀和大鼠棉球植入致肉芽肿等实验，研究其抗炎作用；采用酵母粉致热大鼠模型，研究其解热作用；通过测定大鼠白细胞数和免疫低下小鼠免疫器官质量及碳粒廓清实验，研究其免疫作用，并与忍冬进行比较。结果 灰毡毛忍冬对6种常见致病菌具有显著的抑菌和杀菌作用，MIC为7.8～62.8 mg/mL，MBC≥250 mg/mL；灰毡毛忍冬（10 g/kg）能显著降低小鼠腹腔毛细血管通透性、大鼠足肿胀度和减少肉芽组织的增生，有抑制小鼠耳肿胀和降低发热大鼠体温的趋势，显著提高小鼠脏器指数、廓清指数（K）、吞噬指数（α），有增加大鼠白细胞数的趋势。灰毡毛忍冬与忍冬的上述药效学指标无显著差异。结论 灰毡毛忍冬具有抗菌、抗炎作用和解热的趋势，能增强动物非特异性免疫功能，作用强度与忍冬相当。

Preparation and identification of linarin reference substance from Cirsium setosum

Objective To investigate the preparation method and identification technique of linarin reference substance from Cirsium setosum. Methods The 70% ethanol extract of C. setosum was isolated and purified with petroleum ether defatting, alkali dissolution and acid deposition. Structural identification was carried out by UV, 1H-NMR, and 13C-NMR, and purities were determined by TLC and HPLC methods. Results Linarin reference substances in C. setosum were isolated and purified. There were no impurity spots by TLC check, and the mass fraction of HPLC detection was up to 97%. Conclusion The linarin reference substance prepared by this method complies with the relevant requirements of the chemical markers in Chinese materia medica (CMM), and could be used as chemical reference substance of C. setosum and quality control for its preparations, as well as the study on the pharmacodynamic material basis of CMM.