| 侗族人群成人多囊肾病基因1微卫星DNA多样性分析 |
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| 引用本文： | 苏上贵,赵铖,伍巧源,赖永榕,杨桢华,黎伟.侗族人群成人多囊肾病基因1微卫星DNA多样性分析[J].广西医科大学学报,2007,24(1):26-28. |
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| 作者姓名： | 苏上贵 赵铖 伍巧源 赖永榕 杨桢华 黎伟 |
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| 作者单位： | 1. 广西医科大学生物化学与分子生物学教研室,南宁,530021
2. 广西医科大学第一附属医院内科 |
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| 摘 要： | 目的:了解与成人多囊肾病基因1(PKD1)紧密连锁的微卫星序列DNA等位基因在广西侗族人群中的分布.方法:采用聚合酶链反应(PCR)体外扩增KG8、SM6、CW2和CW4等4个位点的基因片段,用非变性聚丙烯酰胺凝胶电泳,并与标准DNA相对分子质量进行比较分析.结果:在受检的62名侗族人染色体当中,分别在KG8、SM6、CW2和CW4等4个位点分离到了14、12、16和21种等位基因.结论:与PKD1连锁的4个微卫星DNA在广西侗族人群中存在高度多态性.
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| 关 键 词： | 多囊肾病 基因 多态性 |
| 修稿时间： | 2006-07-02 |
THE POLYMORPHISMS OF MICROSATELLITE DNA OF PKD1 IN GUANGXI DONG NATIONALITY POPULATION |
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| Su Shanggui, Zhao Cheng, Wu Qiaoyuan,et al..THE POLYMORPHISMS OF MICROSATELLITE DNA OF PKD1 IN GUANGXI DONG NATIONALITY POPULATION[J].Journal of Guangxi Medical University,2007,24(1):26-28. |
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| Authors: | Su Shanggui Zhao Cheng Wu Qiaoyuan |
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| Institution: | Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning 530021 China |
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| Abstract: | Objective:To study the polymorphism of CW2,CW4 SM6 and KG8,four microsatellite markers closely linked to the locus for polycystic kidney disease(PKD1) in the Guangxi Dong nationality. Methods: To get the gene fragment by using polymerase chain reaction(PCR),and electrophoresis with nondenatured polyacrylamide-gel, then calculate their length separately through linear regression with marker DNA. Result: We have got 16, 21, 12 and 14 kinds of allelic fragments at CW2,CW4,SM6 and KG8 from 120 chromosomes. Conclusion: There are varieties of the allelic at these four sites in the Guangxi Dong nationality. |
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| Keywords: | polycystic kidney decease gene poly-morphisms |
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