Phyllaemblicin B inhibits Coxsackie virus B3 induced apoptosis and myocarditis |
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Authors: | Ya-Feng Wang Xiao-Yan Wang Zhe Ren Chui-Wen Qian Yi-Cheng Li Kitazato Kaio Qing-Duan Wang Yan Zhang Li-Yun Zheng Jin-Hua Jiang Chong-Ren Yang Qing Liu Ying-Jun Zhang Yi-Fei Wang |
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Institution: | a Institute of Pharmacology Science, Jinan University Guangdong, Guangzhou, 510630, China;b National Engineering Research Center of Genetic Medicine, Guangdong, Guangzhou, 510630, China;c Laboratory of Molecular Biology of Infectious Agents, Department of Molecular Microbiology and Immunology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan;d Henan Academy of Medical and Pharmaceutical Sciences, Zhengzhou University, Henan, Zhengzhou, 450000, China;e Kunming Institute of Botany, the Chinese Academy of Sciences, Yunnan, Kunming, 650204, China |
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Abstract: | Coxsackie virus B3 (CVB3) is believed to be a major contributor to viral myocarditis since virus-associated apoptosis plays a role in the pathogenesis of experimental myocarditis. In this study, we investigated the in vitro and in vivo antiviral activities of Phyllaemblicin B, the main ellagitannin compound isolated from Phyllanthus emblica, a Chinese herb medicine, against CVB3. Herein we report that Phyllaemblicin B inhibited CVB3-mediated cytopathic effects on HeLa cells with an IC50 value of 7.75 ± 0.15 μg/mL. In an in vivo assay, treatment with 12 mg kg−1 d−1 Phyllaemblicin B reduced cardiac CVB3 titers, decreased the activities of LDH and CK in murine serum, and alleviated pathological damages of cardiac muscle in myocarditic mice. Moreover, Phyllaemblicin B clearly inhibited CVB3-associated apoptosis effects both in vitro and in vivo. These results show that Phyllaemblicin B exerts significant antiviral activities against CVB3. Therefore, Phyllaemblicin B may represent a potential therapeutic agent for viral myocarditis. |
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Keywords: | Coxsackie virus B3 Viral myocarditis Phyllaemblicin B Antiviral effect Apoptosis |
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