炎症状态下人牙髓神经纤维内Ca^2＋浓度的变化

利用细胞内Ｃａ２＋浓度测定技术，直接测定了牙髓神经纤维内Ｃａ２＋浓度，结果发现：无论是临床炎症性痛过敏牙髓，或是炎症介质组织胺、５－羟色胺处理的牙髓，其神经纤维内Ｃａ２＋浓度均比正常牙髓显著升高。而当降低细胞外液Ｃａ２＋浓度时，虽经炎症介质处理，神经纤维内Ｃａ２＋浓度与正常组无显著差异。说明牙髓神经纤维内Ｃａ２＋升高可能与其炎性痛过敏的发生有关。增加的Ｃａ２＋可能来源于细胞外液的Ｃａ２＋跨膜通道内流

Inflammation-induced Ca 2 increase in nerve terminals in human dental pulp

a 2 concentrations in intradental nerve fibers were measured with micro-fluorophotometry. It was found that either in nerve fibers in the pulp of clinical pulpitis or in nerve fibers treated with 5-HT or histamine, the intracellular Ca 2 concentrations were significantly higher than that in nerve fibers in normal pulp. While in low Ca 2 extracellular solution, no significant difference in intracellular Ca 2 concentration was found between histamine treated and normal groups. The results suggest that the increase of intracellular Ca 2 , as a result of Ca 2 influx, play an important role in intracellular hyperalgesia.