| Pulsinelli四血管法大鼠全脑缺血模型制作方法的改进 |
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| 引用本文： | 李文东,姜洪波,连晓清,刘晓丽,杜爱林.Pulsinelli四血管法大鼠全脑缺血模型制作方法的改进[J].新乡医学院学报,2010,27(3):237-239. |
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| 作者姓名： | 李文东 姜洪波 连晓清 刘晓丽 杜爱林 |
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| 作者单位： | 新乡医学院三全学院; 新乡医学院第三附属医院神经内科; 新乡医学院基础医学院机能学实验室; 新乡医学院基础医学院生理学教研室 |
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| 基金项目： | 新乡医学院高学历人才科研启动项目(2006); 新乡医学院大学生科研创新项目(2007) |
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| 摘 要： | 目的探讨改进大鼠Pulsinelli四血管法全脑缺血模型的制作方法。方法健康雄性SD大鼠60只随机分为对照组10只、假手术组10只和模型组40只。模型组大鼠在Pulsinelli四血管闭塞法全脑缺血模型的基础上采用3阶段制作,第1阶段:游离双侧颈总动脉,备线,缝合皮肤;第2阶段:沿第1颈椎内斜向上的神经来寻找翼突孔,用尖端磨得细长的电烙铁分2次灼烧双侧椎动脉;第3阶段:24 h后夹闭颈总动脉10 min。假手术组除不凝闭椎动脉和不夹闭颈总动脉外,其他操作与模型组相同。对照组不做任何处理。结果大鼠造模成功27只,造模成功率为67.5%,模型制作成功的动物存活率为96.3%。苏木素-伊红染色显示对照组大鼠与假手术组大鼠海马CA1区神经元排列整齐,细胞无水肿、变性;模型组大鼠海马CA1区大量神经元变性坏死。结论采用此改进的手术方法可有效提高大鼠Pulsinelli四血管法全脑缺血模型制作成功率和动物存活率。
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| 关 键 词： | 大鼠 Pulsinelli四血管法 全脑缺血模型 |
Improvement of Pulsinelli four vessels occlusion method in global brain ischemia model of rats |
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| LI Wen-dong,JIANG Hong-bo,LIAN Xiao-qing,LIU Xiao-li,DU Ai-lin.Improvement of Pulsinelli four vessels occlusion method in global brain ischemia model of rats[J].Journal of Xinxiang Medical College,2010,27(3):237-239. |
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| Authors: | LI Wen-dong JIANG Hong-bo LIAN Xiao-qing LIU Xiao-li DU Ai-lin |
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| Institution: | LI Wen-dong~1,JIANG Hong-bo~2,LIAN Xiao-qing~1,LIU Xiao-li~3,DU Ai-lin~4 (1.Grade 2006,Sanquan College,Xinxiang Medical College,Xinxiang 453003,China,2.Department of Neurology,the Third Affiliated Hospital of Xinxiang Medical College,3.Function Laboratory,School of Basic Medicine,4.Department of Physiology,China) |
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| Abstract: | Objective To research the improvement of Pulsinelli four vessels occlusion method in global brain ischemia model of rats.Methods Sixty cases health male SD rats were randomly divided into three groups,the control group(n = 10),the sham-operation group(n = 10) and the model group(n =40).Based on Pulsinelli four vessels occlusion method in global brain ischemia model of rats,the making was done by three steps.First,bilateral common cervical artery was dissociated,cervical artery was threaded and the skin was ... |
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| Keywords: | rat Pulsinelli four vessels method global brain ischemia model |
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