多基因蛋白联合检测在胃癌转移淋巴结中的表达及其相关性

目的研究多基因蛋白在胃癌及其转移淋巴结中的表达水平，探讨其在胃癌转移淋巴结中的临床意义。方法应用免疫组化法检测36例胃癌转移淋巴结中PCNA、C-erbB-2、bcl-2和p16的基因蛋白表达。结果PCNA、c—erbB—2和bcl-2在胃窦部癌转移淋巴结中N1站表达率高于N2、N3站；在各类型胃癌转移淋巴结中N2、N3站表达率呈降低趋势，p16在N3站表达率呈上升趋势；黏液腺癌转移淋巴结中p16表达率最低；分站淋巴结基因表达与原发灶的距离有关。结论联合基因蛋白检测胃癌转移淋巴结可以作为判断术后转移状况和预后的重要参考指标。

Combined polygenic protein examination in lymph node metastasis of gastric carcinoma: expression and relativity

Objective To investigate the gene expression levels of PCNA, C- erbB- 2, bcl- 2 and p16 in lymph node metastasis of gastric carcinoma. Methods The gene expressions of PCNA, C- erbB- 2, bcl- 2 and p16 were examined in 36 cases of lymph node metastasis of gastric carcinoma with the S- P immunohistochemical technique. Results The expression rate of PCNA, C- erbB- 2, and bcl- 2 proved higher in N1 than in N2 and N3 in the lymph node metastasis of gastric- antrum carcinoma, showing a falling tendency in N2 and N3 in various types of lymph node metastasis of gastric carcinoma. The expression rate of p16 showed a rising tendency in N3 and rested at lowest in the lymph node metastasis of gastric muco- adenocarcinoma. Conclusions Combined polygenic protein examination in the lymph node metastasis of gastric carcinoma can make an important reference aid in estimating the postoperative metastasis status and the prognosis.