异种脐带间充质干细胞静脉应用的安全性

背景：人脐带间充质干细胞因其来源丰富，具有较强增殖分化能力，不涉及伦理道德问题，有望成为组织修复与再生工程的首选细胞。但是，对人脐带间充质干细胞异种静脉移植的安全性研究较少。 目的：观察Wistar大鼠静脉输注人脐带间充质干细胞后的安全性。 方法：SPF级Wistar大鼠30只，随机分为空白对照组、阴性对照组、细胞移植组，每组10只，雌雄各半。体外分离扩增人脐带间充质干细胞，细胞移植组大鼠尾静脉一次注射5×106间充质干细胞，注射体积为1 mL；阴性对照组大鼠尾静脉一次注射相同体积50 g/L葡萄糖注射液。注射后每天观察大鼠一般症状，14 d后解剖动物，进行血常规、血生化和脏器病理学检查及脏器质量测定。 结果与结论：细胞移植组大鼠血常规、血生化与对照组比较差异无显著性意义(P > 0.05)。细胞移植组大鼠脏器组织病理学观察与对照组大鼠无明显光镜下形态学差别。结果提示Wistar大鼠一次性静脉移植人脐带间充质干细胞是安全可行的，人脐带间充质干细胞异种移植对受者无不良影响。

Safety of intravenous transplantation of heterogenous umbilical cord mesenchymal stem cells

BACKGROUND: Human umbilical cord mesenchymal stem cells (HUMSCs) can be used as cells for tissue repair and regeneration engineering due to its abundant source, strong proliferation and differentiation ability, uninvolved to ethics problems. However, few studies have addressed the safety of heterogenous HUMSC transplantation via intravenous injection. OBJECTIVE: To study the safety of intravenous injection of HUMSCs in Wistar rats. METHODS: A total of 30 SPF Wistar rats were equally and randomly divided into three groups: blank control, negative control and HUMSC transplantation groups, of equal gender. HUMSCs were isolated and cultured in vitro. Rats in each group were injected via tail vein, with 1 mL of 5×106 HUMSCs as HUMSC transplantation group and 1 mL of 50 g/L glucose as negative control. The rats were observed for 14 days for their general reactions before they were sacrificed. Blood samples were collected for hematological and biochemical studies. Visceral organs were inspected, weighted and studied pathologically. RESULTS AND CONCLUSION: No significant difference in hematological and biochemical studies was detected between HUMSC transplantation group and control group (P > 0.05). No significant difference was determined in histopathological observation in the viscera organs between HUMSC transplantation group and control group. Results have suggested that intravenous transplantation of heterogenous HUMSCs is safe and feasible. No side effects were found in recipients.