| 低氧对人喉鳞癌Hep-2细胞凋亡的影响及其机制探讨 |
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| 引用本文： | 董凯峰,张翠红,宋冬梅,等. 低氧对人喉鳞癌Hep-2细胞凋亡的影响及其机制探讨[J]. 山东医药, 2014, 0(8): 12-13,16 |
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| 作者姓名： | 董凯峰 张翠红 宋冬梅 等 |
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| 作者单位： | [1]河北医科大学第一医院,石家庄050031 [2]白求恩国际和平医院,石家庄050031 |
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| 基金项目： | 河北省医学科学研究重点课题计划项目（20120049）. |
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| 摘 要： | 目的 观察低氧对人喉鳞癌Hep-2细胞凋亡的影响,并探讨其机制.方法 将体外培养的人喉鳞癌细胞分为常氧组、低氧组.流式细胞仪检测细胞凋亡率,RT-PCR及Western blot法分别检测各组细胞低氧诱导因子α（HIF-1α）、赖氨酰氧化酶（LOX）mRNA及蛋白.结果 常氧组细胞凋亡率13.86％±0.12％、低氧组5.13％±0.67％,P≤0.05.与常氧组比较,低氧组细胞中HIF-1 α、LOX蛋白及其mRNA表达增加（P均≤0.05）,且二者表达呈正相关（r均为0.862,P均≤0.05）.结论 低氧可抑制Hep-2细胞凋亡,该作用可能与其上调细胞中HIF-1α、LOX表达有关.
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| 关 键 词： | 喉肿瘤 Hep-2细胞 低氧 凋亡 低氧诱导因子 赖氨酰氧化酶 |
Effect of hypoxia on apoptosis of human laryngeal cancer Hep-2 cell line and its mechanism |
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| Affiliation: | DONG Kai-feng(The First Hospital of Hebei Medical University, Shijianzhuang 050031, China)〈br〉,ZHANG Cui-hong,SONG Dong-mei(The First Hospital of Hebei Medical University, Shijianzhuang 050031, China)〈br〉,LYU Xin(The First Hospital of Hebei Medical University, Shijianzhuang 050031, China)〈br〉,ZHANG Ji-hua(The First Hospital of Hebei Medical University, Shijianzhuang 050031, China)〈br〉,XUE Hai-tao(The First Hospital of Hebei Medical University, Shijianzhuang 050031, China)〈br〉,ZHAO Yu-ling(The First Hospital of Hebei Medical University, Shijianzhuang 050031, China)〈br〉 |
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| Abstract: | Objective To investigate the effect of hypoxia on apoptosis of human laryngeal cancer Hep-2 cell line and its mechanism.Methods Human laryngeal squamous carcinoma cells cultured in vitro were divided into the normoxic,and hypoxic groups.The apoptosis was detected by flow cytometry,and the expression of hypoxia-inducible factor （HIF）-1 a,lysyl oxidase （LOX） protein and mRNA was detected by Western blot and RT-PCR,respectively.Results Hypoxia significantly increased the expression of HIF-1 a protein and mRNA in the Hep-2 cells and the LOX protein and mRNA expression was up-regulated.Flow cytometry showed that the apoptosis rates were （13.86 ± 0.12） ％ in the normoxic group and （5.13 ± 0.67）％ in the hypoxia group,statistically significant difference was found between the two groups （P ≤0.05）.Conclusion Hypoxia inhibits the apoptosis of Hep-2 cells,whose mechanism may be associated with the up-regulation of HIF-1a and LOX expression. |
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| Keywords: | laryngeal neoplasms Hep-2 cells hypoxia apoptosis hypoxia-inducible factor lysyl oxidase |
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