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| HPLC法同时测定雅静胶囊中厄贝沙坦和氢氯噻嗪的含量 | |
| 引用本文： | 吴佳琪,郭启智,江春霞. HPLC法同时测定雅静胶囊中厄贝沙坦和氢氯噻嗪的含量[J]. 中国药师, 2009, 12(4): 464-466 |
| 作者姓名： | 吴佳琪 郭启智 江春霞 |
| 作者单位： | 1. 上海市交通大学附属第一人民医院药学部,上海,200080 2. 上海长征医院心内科 3. 上海长征医院药学部 |
| 摘 要： | 目的：建立HPLC法测定雅静胶囊中厄贝沙坦和氢氯噻嗪的含量。方法：色谱柱：Dianmonsil TMC18柱（250mm×4．6mm，5μm）；流动相：乙腈-0．08mol·L^-1磷酸溶液（用三乙胺调至pH5．0）（40：60）；流速：1ml·min^-1；紫外检测波长：225nm；进样量：20μl；柱温：25℃。结果：厄贝沙坦和氢氯噻嗪分别在15～135μg·ml^-1（r=0．9999）和1．2～10．8μg·ml^-1（r=0．9999）浓度范围内线性关系良好，平均回收率分别为100．0％和100．5％。结论：本方法操作简便、快捷，重复性好，灵敏度高，结果准确可靠，适用于雅静胶囊的质量控制。 |
| 关 键 词： | 厄贝沙坦 氢氯噻嗪 雅静胶囊 HPLC |
Simultaneous Determination the Contents of Irbesartan and Hydroehlorothiazide in Yajing Capsules by HPLC |
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| Wu Jiaqi,Guo Qizhi,Jiang Chunxia. Simultaneous Determination the Contents of Irbesartan and Hydroehlorothiazide in Yajing Capsules by HPLC[J]. China Pharmacist, 2009, 12(4): 464-466 | |
| Authors: | Wu Jiaqi Guo Qizhi Jiang Chunxia |
| Affiliation: | Wu Jiaqi, Guo Qizhi, Jiang Chunxia( 1. Department of Pharmacy, Affiliated First People＇s Hospital, Shanghai Jiaotong University, Shanghai 200080, China; 2. Department of Heart Medicine, Shanghai ChangZheng Hospital; 3. Department of Pharmacy, Shanghai ChangZheng Hospital) |
| Abstract: | Objective： To establish an HPLC method for determination of irbesartan and hydrochlorothiazide in Yajing capsules. Method： A DianmonsilTM C18 column （ 250 × 4.6mm, 5 μm） was used with the mobile phase of acetonitrile - 0.08 mol· L^-1 phosphoric acid （adjust pH 5.0 with triethylamine） （40：60）, and detection wavelength was at 225 nm. Result： The linear ranges of irbesartan and hydrochlorothiazide were 15 - 135 μg· ml ^-1 （ r = 0. 999 9） andl. 2 - 10.8 μg· ml^- 1 （ r = 0.999 9 ）, respectively. The average recoveries were 100.0% and 100.5 %, respectively. Conclusion： The method is simple, feasible and repeatable. It has supplied theoretical evidence for quality control of Yajing capsules. |
| Keywords: | HPLC |
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