高效液相色谱法同时测定金银花中绿原酸和木犀草苷的含量*

目的]采用梯度洗脱方法建立金银花中绿原酸和木犀草苷含量测定的高效液相色谱(HPLC)法。方法]色谱柱:Xterra@RP185;检测器:Waters2998二级管阵列检测器(DAD);工作站:Waters Empower;流动相:0.5%冰乙酸-乙腈;线性梯度洗脱0:～15 min(90∶10-80∶20),15～30 min(80∶20-75∶25),30～40 min(75∶25-65∶35),40～45 min(65∶35-90∶10);流速:1 mL/min;检测波长:350 nm。结果]在此色谱条件下两种成分分离完全。结论]该方法简便,准确,快速易行,可同时检测金银花中两种成分的含量。

Simultaneous determination of luteolin-7-O-glu-coside and chlorogenic acid in Flos Lonicerae Japonicae by HPLC

Objective] To establish a HPLC method for quantitative determination of luteolin-7-O-glu-coside and chlorogenic acid in Flos Lonicerae Japonicae using gradient elution method.Methods] Determining column: Xterra@ RP 185;Detector: Waters 2 998 diode array detector(DAD);Workstation: Waters Empower;The mobile phase: 0.5% acetic acid-acetonitrile;Linear gradient elution: 0~15min(90 ∶ 10-80 ∶ 20),15~30 min(80 ∶ 20-75 ∶ 25),30~40 min(75 ∶ 25-65 ∶ 35),40~45 min(65 ∶ 35-90∶ 10);The flow rate was 1 mL/min,and the detection wavelength was set at 350 nm.Results] In this chromatographic conditions,the two components were completely separated.Conclusion] The method is simple,accurate,fast and easy,which can detect two components of Flos Lonicerae Japonicae simultaneously.