| 甘精、地特、人胰岛素对3T3-L1脂肪细胞脂联素mRNA表达的影响 |
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| 引用本文： | 梁玉,杨静,阴津华.甘精、地特、人胰岛素对3T3-L1脂肪细胞脂联素mRNA表达的影响[J].中国现代医生,2011,49(13):10-12. |
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| 作者姓名： | 梁玉 杨静 阴津华 |
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| 作者单位： | 山西医科大学第一临床医学院内分泌科,山西太原,030001 |
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| 摘 要： | 目的探讨甘精、地特、人胰岛素对3T3-L1脂肪细胞脂联素mRNA表达的影响。方法体外培养3T3-L1脂肪细胞，诱导分化成熟后加入含有1nmol／L、10nmol／L、100nmol／L、1000nmol／L甘精、地特、人胰岛素的基础培养基培养24h，并设立基础培养基组为空白对照组，培养24h，RT-PCR方法检测脂肪细胞脂联素mRNA水平。结果100nmol／L、1000nmol／L甘精、地特、人胰岛素均抑制脂联素mRNA的表达中，1000nmol／L胰岛素的抑制作用强于100nmol／L胰岛素。地特胰岛素的抑制作用弱于人胰岛素及甘精胰岛素。甘精胰岛素的抑制作用弱于人胰岛素。结论高浓度甘精、地特、人胰岛素均不同程度抑制3T3-L1脂肪细胞脂联素mRNA的表达。
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| 关 键 词： | 甘精胰岛素 地特胰岛素 人胰岛素 脂联素 3T3-L1脂肪细胞 |
Regulation of Adiponectin mRNA Expression by Glargine,Detemir and Human Insulin in 3T3-L1 Adipocytes |
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| Authors: | LIANG Yu YANG Jing YIN Jinhua |
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| Institution: | LIANG Yu YANG Jing YIN Jinhua Department of Endocrinology,First Clinical Medical College of Shanxi Medical University,Taiyuan 030001,China |
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| Abstract: | Objective To investigate the effect of glargine,detemir and human insulin on the expression of adiponectin mRNA in 3T3-L1 adipocytes.Methods 3T3-L1 adipocytes were cultured in vitro until they were differentiated into mature adipocytes,then they were cultured for 24 hours in basal medium which containing of 1nmol/L,10nmol/L,100nmol/L,1000nmol/L glargine,detemir and human insulin,and established basal medium group for control group,after 24 hours,the level of adiponectin mRNA in adipocytes were measured by R... |
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| Keywords: | Insulin glargine Insulin detemir Human insulin Adiponectin 3T3-L1 adipocyte |
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