超微藿香正气散对急性腹泻大鼠肠黏膜保护及水电解质的调节作用研究

目的探讨超微藿香正气散对急性腹泻大鼠肠黏膜保护和水电解质的调节作用。方法 50只大鼠随机分为空白组、模型组、超微粉组、细粉组、口服液组。采用番泻叶煎液加寒冷刺激灌胃复制大鼠急性腹泻模型,观察大鼠腹泻次数、粪便含水量的变化,用光镜观察大鼠结肠HE染色黏膜形态学变化,电极法检测血清K+、Na+、Cl-浓度,酶联免疫法检测血浆环磷酸腺苷(cAMP)水平。结果模型组大鼠腹泻次数、粪便含水量明显增加,血浆Na+、K+浓度降低、Cl-浓度升高,血浆cAMP水平升高,超微粉、细粉、口服液组与模型组比较,差异有统计学意义(P<0.05);超微粉组与细粉组比较,在降低大鼠腹泻次数、粪便含水量,升高血浆Na+、K+浓度,降低血浆Cl-浓度、cAMP水平方面差异有统计学意义(P<0.05);超微粉组与口服液组比较,在降低大鼠腹泻次数方面差异有统计学意义(P<0.05)。结论超微藿香正气散能调节急性腹泻大鼠水电解质平衡,降低血浆cAMP水平,对肠黏膜有保护作用,其作用优于藿香正气散细粉。

Effects of micro-pieces Huoxiang Zhengqi powder on protection of intestinal mucosa and regulation of water electrolyte in rats with acute diarrhea

Objective To investigate the effect of micro-pieces Huoxiang Zhengqi powder on protection of intestinal mucosa and regulation of water electrolyte in rats with acute diarrhea.Methods 50 rats were randomly divided into blank group,model group,micro-pieces powder group,powder group and oral solution group.The gavage senna decoction combined with cold stimulation was used to induce the rat models,the changes of diarrhea times and water content in fecal were measured,the HE staining colon mucosa morphology was detected with light microscopy,the serum concentration of K+,Na+ and Cl with electrode and plasma level of cAMP with enzyme-linked immunosorbent.Results In model group,the diarrhea times and fecal water content were significantly increased,plasma K+,Na+ concentration decreased,Cl concentration and plasma cAMP levels increased;compared micro-pieces powder group,powder group and oral solution group with model group,the differences were significant(P<0.05);compared superfine powder group with the powder group,the differences of decreasing diarrhea times and fecal water content,increasing plasma K+,Na+ concentration,decreasing plasma Cl-concentration and cAMP levels were significant(P<0.05);compared superfine powder group with oral solution,the difference of reducing diarrhea times was significant(P<0.05).Conclusion The micro-pieces Huoxiang Zhengqi powder can regulate water and electrolyte balance in rats with acute diarrhea,decrease plasma cAMP levels,is effective to protect the intestinal mucosa,and is better than traditional powder.