| 高效液相色谱一质谱联用法测定人血浆中非索非那定的浓度 |
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| 引用本文： | 张洪,李健和,朱运贵,王峰,易利丹.高效液相色谱一质谱联用法测定人血浆中非索非那定的浓度[J].中国医药导报,2011,8(22):28-30. |
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| 作者姓名： | 张洪 李健和 朱运贵 王峰 易利丹 |
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| 作者单位： | 1. 湖南省湘西自治州人民医院药剂科,湖南湘西,416000
2. 中南大学湘雅二医院药学部,湖南长沙,410011 |
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| 摘 要： | 目的：建立以高效液相色谱-质谱联用法测定人血浆中非索非那定浓度的方法。方法：以苯乙双胍为内标物,采用C18柱,流动相由乙腈及30 mmol/ml NH4Ac（含0.1%甲酸,0.01%三氟乙酸）组成,采用梯度洗脱程序,测定血浆中非索非那定浓度。结果：非索非那定在2.26～676.46 ng/ml线性关系良好（r=0.999 6,n=7）,定量下限浓度为2.26 ng/ml,RSD为0.7%,高、中、低浓度测定的相对回收率分别为（109.9±1.7）%、（103.1±7.2）%和（101.5±13.4）%,日内RSD分别为2.1%、2.5%和9.8%,日间RSD分别为1.3%、5.7%和7.1%。结论：所建方法预处理简单﹑专属性强、灵敏度高,适合测定非索非那定的血药浓度。
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| 关 键 词： | 非索非那定：血药浓度 LC-MS／MS |
Determination of Fexofenadine in human plasma by LC-MS/MS |
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| ZHANG Hong,LI Jianhe,ZHU Yungui,WANG Feng,YI Lidan.Determination of Fexofenadine in human plasma by LC-MS/MS[J].China Medical Herald,2011,8(22):28-30. |
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| Authors: | ZHANG Hong LI Jianhe ZHU Yungui WANG Feng YI Lidan |
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| Institution: | 1.Department of Pharmacy,the People′s Hospital of Xiangxi Autonomous Perfecture,Xiangxi 416000,China;2.Department of Medicine,the Second Xiangya Hospital of Central South University,Changsha 410011,China |
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| Abstract: | Objective: To establish the LC-MS/MS method for the determination of fexofenadine in human plasma.Methods: Phenformin was used as internal standard.C18 column was used with the mobile phase of acetonitrile and 30 mmol/mlNH4Ac(containing 0.1% formic acid and 0.01% trifluoraceticacid).The concentration of fexofenadine in human plasma was determined by the method of LC-MS/MS with gradient elution.Results: A linearity was obtained from 2.26 to 676.46 ng/ml of fexofenadine in human plasma with a good correlation... |
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| Keywords: | LC-MS/MS |
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